Báo cáo khoa học: " Loratadine dysregulates cell cycle progression and enhances the effect of radiation in human tumor cell lines" pot

... [3], loratadine alone results in Chk1 activation leading to an increase in the percen-tage of cells in the G2/M phase of the cell cycle. Since the G2/M phase of the cell cycle is one of the most ... determinedby dividing the density of the released DNA band by the density of the total DNA in the l ane (the released DNAband plus the unreleased DNA remaining in the well). Cell Cycle Analysis The ... to radiation alone, the loratadine- treated irradiated cells hadreturned to a more normal cell cycle distribution within18 hours of the removal of loratadine. Effect of Loratadine on Cell Cycle- associated...

Tài liệu Báo cáo khoa học: Long-distance interactions between enhancers and promoters The case of the Abd-B domain of the Drosophila bithorax complex pdf

... the effects of the neighboring chromatin (e.g. heterochro-matinization) if the reporter is flanked by two of them. The apparent function of the boundaries within BX-Cis to separate neighboring ... [11].However, these effects are observed only if at least onecopy of them is present in a transgenic insert, and the significance of this high affinity pairing in the regula-tion of the Abd-B is therefore ... pairing between these sequences within the BX-C plays a role in restricting the extent of loop-ing-out domains.Tethering elementsDeletion analysis of the Abd-B gene strongly suggests the existence...

Báo cáo khoa học: Cyclic ADP-ribose requires CD38 to regulate the release of ATP in visceral smooth muscle ppt

... suggesting that the two adenine nucleotides may work in synergy to maintain the restingtone of the bladder. In conclusion, extracellular cADPR enhances the spontaneous release of ATP in the bladder ... preparation. Of particular importance is the ﬁnding that exogenous cADPR failed to increase the spontaneous release of ATP in the absence of CD38. In other words, the presence of CD38 is mandatoryfor the ... suggesting that prejunction-al P2 receptors were not involved in the facilitatingeffects of cADPR. In contrast, the inhibitors of intra-cellular cADPR receptors 8-Br-cADPR (80 lm) and ryanodine...

báo cáo khoa học: "Gastrointestinal Stromal Tumours treated before and after the advent of c-kit immunostaining" pptx

... (GUMP, GIST, GANT, and now GIPACT). Implications of c-kit in genesis, and yet another of many emerging roles of the interstitial cell of Cajal in the pathogenesis of gastrointestinal disease. Adv ... expression of the c-kitreceptor (CD117), as is their cell of origin, the intersti-tial cell of Cajal [2]. Detection of the c-kit protein in tumour cells by immunohi stochemistry is now the stan-dard ... bleeding, apalpable mass, obstruction or abdominal pain. The aim of our study was to review the clinicopatho-logical features of previously resected mesenchymaltumours of the GI tract in our institution...

Tài liệu Báo cáo khoa học: Erythrochelin – a hydroxamate-type siderophore predicted from the genome of Saccharopolyspora erythraea docx

... the C-terminal C5-domain,catalyzing the intramolecular nucleophilic attack of the L-hOrn3a-amino group onto l-haOrn4. Takinginto account that the synthetases involved in the bio-synthesis ... enterobactin and coelichelin [6,7]. The C-terminal adenylation domainA4again is predicted to activate l-arginine, displaying60% identity to the characterized A-domain of MycC.Interestingly, A1 and ... encoded in the etc gene cluster. The N-terminal C-domain of the NRPS catalyzes the a-N-acetylation of haOrn1 in cis.Cyclorelease of the assembled tetrapeptide mediated by the C-terminal C-domain of...

Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc

... Leadeterminant.As the C3aion is at m/z 690, this excludes the possibility of the other Fuc being at the Gal next to the subterminalGlcNAc. The position of the second Fuc 3-linked to the internal ... function.One of the early demonstrations of the role of carbo-hydrates in recognition was binding of the in uenza virus tored blood cells via sialic acid [1], and later by work on the chemical basis of ... containing a Fuc at the ﬁrstGlcNAc residue of the 6-branch forming an internal Lexepitope, and the trifucosylated iso-lacto-N-octaose, contain-ing two terminal Leaepitopes at each of the...

Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

... for example, in order to save energy and protein synthesizing capa-city. This would be preferable to placing all the genesinvolved in the pathway in the same operon; the cell can then respond ... expres-sion of the las operon around the normal level led tolittle change in the production pattern. By coregulatingPK and LDH cells can maintain homolactic fermenta-tion. The fact that the effects of ... for the purpose of determining relative activities. The speciﬁc activ-ities of PFK and PK and LDH in MG1363 were deter-mined as UÆmg)1 of protein, where a unit (U) is deﬁned as the amount of...

Báo cáo khoa học: Mycobacterium tuberculosis possesses a functional enzyme for the synthesis of vitamin C, L-gulono-1,4-lactone dehydrogenase doc

... including: biosynthesis of colla-gen and other hydroxyproline ⁄ hydroxylysine-containingproteins ⁄ peptides; synthesis of secondary metabolites,hormones and cytokines [1]; oxidative protein ... protein band, thus pointing to the absence of a covalently bound ﬂavin moiety in the recombinant product. Moreover, as in the case of the plant l-galactono-1,4-lactone dehydrogenase [12], the ... Escherichia coli. The recombinantprotein contained an engineered enterokinase cleavagesite in the junction between the GST tag and the Rv1771 sequence. Upon 3 h of induction of the pDEST15_Rv1771...

Báo cáo khoa học: Interaction between catalytically inactive calpain and calpastatin Evidence for its occurrence in stimulated cells docx

... concentra-tions of the metal ion [45]. The addition of E64, a syn-thetic inhibitor of calpain, did not modify the intensity of the signal observed in the presence of Ca2+alone. The increase in the mAb-binding ... by evaluating the intensity of the light signal generated by the binding of mAb56.3.To perform these investigations, the two preferentialligands of calpain, Ca2+ions and calpastatin, were ... calpain staining increased 7–8-fold, indicating that the protease was now mainlypresent in the high-afﬁnity form. In these conditions, although the highest amount of calpain is still in the...

Báo cáo khoa học: A ‘pure’ chemoattractant formylpeptide analogue triggers a speciﬁc signalling pathway in human neutrophil chemotaxis potx

... phosphatidylinositol-3-kinase, as well asactivating tyrosine phosphorylation. An increase in intracellular levels of cAMP [6,7] and the involvement of kinases, such as protein kinase C (PKC) and mito-gen ... regions including talin, vinculin and integrins (focal adhesions). Within these regions,several components of the cytoskeleton are concentra-ted, together with a number of signalling proteins. ... withb-integrin vesicle trafﬁcking [27] and therefore the linkbetween PKC b and b2integrins may not be coinciden-tal. The genes encoding PKC b1isoform, leukocyteadhesion receptor (CD43) and...

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