... separated bands, TLC plates were dippedinto a charring solution consisting of 0.63 g of MnCl2Æ4H2O,60 mL of water, 60 mL of methanol and 4 mL of concen-trated sulfuric acid, briefly dried and ... arginine at the extreme N-terminus, istargeted for degradation by the enzymes of the N-end rule. A pro-tein–protein interaction therefore results in nongrowth on mediumlacking uracil. (B) In ... at G1 and theformation of a mating projection following pheromonestimulation [18,20], and Cla4p also seems to play aminor role in this pheromone signalling [56,57], butneither protein seems...