... Take, M., Tsutsui, J., Obama, H., Ozawa, M., Nakayama, T.,Maruyama, I., Arima, T. & Muramatsu, T. (1994) Identificationof nucleolin as a binding protein for midkine (MK) and heparin-binding ... nucleolin was generated by PCR amplificationusing total first-strand cDNA as template and the followingoligonucleotides: 5¢-TGGTATGACTAGGAAATTTGGTTATGTG-3¢ and 5¢-GACAGAAGCTATTCAAACTTCGTCTTC-3¢. ... retained on an affinity matrixcontaining purified Lf. In view of this and of a previousobservation pointing out that nucleolin serves as a bindingprotein for various ligands, we investigated...