... forquantitation of microgram quantities of protein utilizingprinciple of protein-dye binding. Anal Biochem 72, 248–254.34 Tang D, Shafer MM, Vang K, Karner DA & Arm-strong DE (2003) Determination of ... (black line, A,B,C), fitting a single-enzyme model (blue line, A), fitting a two-enzyme model (red line, B) and fitting asingle-enzyme model with competitive prod-uct inhibition (yellow line, C). ... controlling the intracellular concentration of methylglyoxalare its formation rate and the concentration of trypanothione. Glyoxalase Iand II activities play only a minor role in maintaining a...