... 0) of the BuChE-catalysed hydrolysis of both substrates: Ki= 0.49 ±0.05 mm with ASCh, and Ki= 0.74 ± 0.58 mm with o-NTFNAC. Thus, propanil interferes with the forma-tion of ES, but not with ... gorge of D70G compared with that of the wild-type enzyme for acylation with neutral ester.Effects of benzalkonium on ATMA hydrolysisHydrolysis of ATMA by wild-type BuChE in the pres-ence of increasing ... clearly rule out this hypothesis. First, kinetic analysis of organophosphate inhibition of the esterand AAA activities of BuChE indicates that there is asingle nucleophilic serine, Ser198, for...