... after radiation for total TGF-β1, as well as for active TGF-β1, with commer-cially available kits (Promega, Madison, WI). Measure-ments of active and total amounts of TGF-β1 wereperformed in separate ... active fraction of TGF-β1was assayed directly in the ELISA plate using the kits pro-vided. For measuring the total amount of TGF-β1, addi-tional samples were acidified to pH 3.0 using 1 mol/LHCl, ... http://www.ro-journal.com/content/3/1/40Page 7 of 7(page number not for citation purposes)9. Green MC, Sweet HO, Bunker LE: Tight-skin, a new mutation of the mouse causing excessive growth of connective tissue andskeleton....