Báo cáo khoa học: " Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response" potx

... of 13RESEARCH Open Access Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage responsePei-Ming ... design DNA- directed alkylating agents by linking the alkylating pharmacophore to the DNA- affinity molecules (e.g., DNA intercalating agents, DNA minor groove binder)[7,8]. In most cases, the DNA- directed ... article as: Chu et al.: Enhancement of radiosensitivity in human glioblastoma cells by the DNA N-mustard alkylating agent BO-1051 through augmented and sustained DNA damage response. RadiationOncology...

Tài liệu Báo cáo khoa học: Altered deoxyribonucleotide pools in T-lymphoblastoid cells expressing the multisubstrate nucleoside kinase of Drosophila melanogaster doc

... transduc-tion in both cell lines (data not shown). In order to test the enzymatic activity of the Dm-dNK–GFP fusion protein and the level of nucleo-side kinase activity in the cells, we determined the ... retainedenzymatic activity in these human T-cell lines.Increasing sensitivity to nucleoside analogs in Dm-dNK expressing cells We determined the sensitivity of the untransduced H9 and CEM cells ... available for the DNA synthe-sis machinery [2]. It is essential that the concentration of each dNTP is maintained in proportion to the abundance of the different nucleotides in the DNA. Unbalanced...

Báo cáo khoa học: Expression of cholinesterases in human kidney and its variation in renal cell carcinoma types pdf

... erythrocytes, and the difference between acetylcholinesterases of kid-ney and erythrocytes in the extent of binding with the lectins concanavalin A, Lens culinaris agglutinin(LCA), and Ricinus communis ... mRNA, and the b-actin mRNA, used as the internal standard. The sequence and position of the prim-ers, as well as the size of the PCR products, are provided in Fig. 5. cDNA was ampliﬁed in an ... in the human urothelium [34], the expression of cholinesterases in human kidney has notbeen studied yet. The present research was intended topartially ﬁll this gap by examining the levels of...

báo cáo khoa học: " Regulation of hypoxia inducible factor-1a expression by the alteration of redox status in HepG2 cells" pdf

... kit(Invitrogen, USA). The quantity and quality of RNA wasdetermined by the OD measurement at 260 and 280nm. The integrity of RNA was checked by visual inspec-tion of the two rRNAs 28S and ... normalcondition, the majority of glutathione is in the reducedform. Shifting redox equilibrium is in favor of a redu-cing or oxidizing state; that is in modification of the redox status in cells [22,23]. The ... scavenging of the most of ROS and free radicals in hypoxic cells, but abit of ROS generation from mitochondria possiblyinduced the expression of HIF-1a [33].To further judge our finding, the...

Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc

... increase of GTPcS binding was completely inhibited by Fig. 2. Binding of muscarinic ligands. Allexperiments were performed in triplicate.Each data point represents the mean ± SEM.(A) Binding ... over the detection sensitivity in the western blot analysis and well transferred in the membrane fraction.Binding properties of muscarinic ligands withM2::GOA-1 fusion protein The binding ... GTPcS by GDP was3 · 10)6m with 0.2 mm atropine, and 10)4m withoutatropine in the presence of 1 mm carbamylcholine,which induced the maximum increase in GTPcS bind-ing (Fig. 3B), suggesting...

Báo cáo khoa học: "MicroRNA expression profiles in human cancer cells after ionizing radiation" pps

... analyzed, the changes observed allow the generation of hypotheses and the design of further experiments validating the initial findings presented here and investigating the functional relevance of microRNA ... predict the efficacy of therapeutic interventions, including radiother-apy [8,9]. In fact, microRNAs have been shown to mo d-ulate the radiosensitivity of lung cancer cells in vitro[10] and bre ... miR-16 induce apoptosis by targeting BCL2. Proceedings of the National Academy of Sciences of the United States of America 2005, 102:13944-13949.5. Volinia S, Calin GA, Liu CG, Ambs S, Cimmino...

Tài liệu Báo cáo khoa học: Enhancement of oxidative stress-induced apoptosis by Hsp105a in mouse embryonal F9 cells pptx

... prominently observed by Hoechst 33342 staining in these cells (Fig. 3A), and the rate of apoptotic cells was approximately threefoldhigher in the Hsp105a over-expressing cells than in F9or V1 cells ... determine the effects of Hsp105a on JNK and p38 signalling pathways, weexamined the activation of JNK and p38 in F9 cells by oxidative stress. As shown in Fig. 6A, JNK was notactivated in control ... or V1 cells by treatment withhydrogen peroxide. In contrast, hydrogen peroxide-treat-ment induced marked activation of JNK within 30 min in S23 cells, but not in S3 cells. Therefore, the enhancement...

Báo cáo khoa học: " Enhancement of protective immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA in mice" pptx

... assessed by routine staining with hematoxylin and eosin, and examined by light microscopy. The inflammatory response was evaluated by examining the lung tissue for the presence of typical in- flammatory ... immune system by interacting with intestinal epithelial cells in the presence of butyric acid, a metabolite produced by intestinal bac-teria [29]. In addition to the induction of a specific ... infections by decreasing mortality. It was also possible that IgA contributed to the protective mechanism by inhibiting the entrance of the pathogen into the lung and by modulating the pro -in- flammatory...

Tài liệu Báo cáo khoa học: "ANALYSIS OF OONOUNCTIONS IN PAKSER" potx

... phases of the interpretation of the sentence "Bob met Sue and Mary in London". H means "head" and indicates the position of the node filler within the sequence of dependent ... new pieces of information, which were implicit in the "linear" input form, are made explicit in the result of the analysis. These pieces of information are extracted using the syn- ... are two operators: CRLINK (X,Y) which creates a new instance of the type X and links it to the nearest node of type Y existing in the rightn~Dst path of the tree (and moving only upwards) FILL...

Báo cáo khoa học: "Role of Verbs in Document Analysis" pot

... goal of our research is to identify the role of verbs, keeping in mind that event profile is but one of many factors in determining text type. In our study, we explored the contribu- tion of ... they were commonly post- ings of company earnings reaching a new high or dropping from last quarter. Agreement and argument verbs appeared in many of the same articles, involving issues of ... discrim- ination. Although the WordNet results were not discriminatory, we were still convinced that our initial hypothesis on the role of verbs in determining event profile was worth pursuing....

Xem thêm