... ACKNOWLEDGEMENTSThis work was supported in p art by Grant-in-aids for Scienti®cResearch from the Ministry of Education, Science, Sports, and Cultureof Japan ... clones generated by PCR from the spermosin c DNA as described in Mate-rials and methods. GST±spermosin lightchain fusion proteins (3 lg each), which hadbeen previously puri®ed by glutathione±agarose ... corresponding to the L1, L2, and L1(DL2 ),which is an L1 lacking L2 region, were ampli®ed by PCR from the cDNAs using the following combinations offorward and reverse p rimers designed to produce 5¢ BamHIand...