Báo cáo khoa học: "Intraoperative frozen section assessment of sentinel lymph nodes in the operative management of women with symptomatic breast cancer" ppt

Tài liệu Báo cáo khoa học: Accessibility changes within diphtheria toxin T domain when in the functional molten globule state, as determined using hydrogen⁄deuterium exchange measurements pdf

... catalytic domain. Here, the data allowed identiﬁcation of the core of the pro-tein in the N state and the evolution of the overallstructure of the protein in the MG state. This degree of resolution ... furin into two fragments: the A chaincorresponding to the catalytic domain (C domain);and the B chain corresponding to the translocationdomain (T domain) and the receptor-binding domain. The ... isinvolved in the dimer interface. TH9 can be consideredas involved in the protein core but to a lesser extent.Interestingly, from these results, the current view of the core of the T domain...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia histone methylases play critical roles in estrogen-mediated regulation of HOXC13 docx

... and ERb wasincreased in both ERE1 and ERE2 in the presence of E2. Interestingly, the kinetics of E2-dependent binding of ERa and ERb to both ERE1 and ERE2 are differ-ent. The binding of ERa is ... absence of E2, and this binding wasincreased in the presence of E2 (Fig. 5A,B; compare0 h and 6–8 h time points). These differences in the kinetic proﬁles of binding of ERa and ERb suggestthat they ... wasobserved in any of the EREs, irrespective of the absence and presence of E2. Binding of ERa and ERbwas increased in both ERE1 and ERE2 of the HOXC13 promoter (Fig. 5A, lanes 1–4). The levels of E2-induced...

Tài liệu Báo cáo khoa học: What makes biochemical networks tick? A graphical tool for the identiﬁcation of oscillophores ppt

... cycles in such a combinationdo not touch each other). The value (the sign) of a cycle is the product of the values (the signs) of its steps. The sign o f the magnitude of a Ôminus combined autoinfluence ... The absolute magnitude of acombined autoinfluence path is the product of all the ratesdivided by the product of all the concentrations of itsspecies. Therewith the c oefficient of o rder p of ... identifysubschemes of the specific biochemical network that induceinstability. We then consider inte rconnections between sucha subscheme a nd other parts of the kinetic scheme with orwithout eliminating the...

Tài liệu Báo cáo khoa học: Casein kinase 2 speciﬁcally binds to and phosphorylates the carboxy termini of ENaC subunits ppt

... presence of the a subunit of CK2 in the active cytosolic fractions(Fig. 6), and the ﬁnding that the electrophoretic mobility of the phosphorylating kinase corresponds to that of the asubunit of CK2 ... and2Department of Biological Regulation, The Weizmann Institute of Science, Rehovot, IsraelA number of ﬁndings have suggested the involvement of protein phosphorylation in the regulation of the epithelialNa+channel ... expression of ENaC is determined by an interaction between the C tails of b and c and the ubiquitin ligase Nedd4. The WW domains of Nedd4 bind to the proline-rich PY motifs of b andcENaC, leading to...

Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf

... was incu-bated with insulin for 30 min in the presence of dithiothreitol, and solution turbidity was measured at 400 nm. The p26 variants tested areindicated in the ﬁgure and they appear in the ... domain. The inter-face between monomers of a dimer involves interactionbetween strands b2 and b6 of neighboring monomers. The p26 modiﬁcations examined in this study are notlocated in either of these ... 1023C-terminal extensions which extend to neighboringmonomers, and the remaining b-strands, with the exception of strand 6, are arranged in two antiparallelbeta sheets within the a-crystallin domain....

Báo cáo khoa học: Structural evidence for a constant c11 ring stoichiometry in the sodium F-ATP synthase doc

... c in the E. coli host cells, indicating that intrinsic fea-tures of the monomeric unit determine the number of subunits that can be packed into the ring. These dataare fully compatible with the ... treatment. The observed accumulation of the incomplete c10complex in the recombinant c ringpreparations suggests that the insertion of the last csubunit forms the limiting step in the assembly ... into the c11rings in the presence of SDS. The second type of aggregate appears as aladder above the original c11band on SDS ⁄ PAGE andconsists of c11rings hosting varying amounts of the...

Báo cáo khoa học: a-1 Antitrypsin binds preprohepcidin intracellularly and prohepcidin in the serum pdf

... preprohepci-din interacted with the mature form of hepcidin in BacterioMatch screening. This further supports the possibility that these proteins may play a role in the protection of prohepcidin from the ... and within 5% of each other. For the relative gene expression analysis, the 2DDCt(Livak)method was used. The expression level of the gene of interest was compared with the level of b-actin in ... hepcidin is with the iron exporter molecule ferroportin. We wereinterested in whether we could identify new protein–protein interactions of preprohepcidin, prohepcidinand hepcidin in vivo. For these...

Báo cáo khoa học: A novel gene, fad49, plays a crucial role in the immediate early stage of adipocyte differentiation via involvement in mitotic clonal expansion docx

... found in the FAD49 PXdomain, as in many PX domains, suggesting that the PX domain could bind to an SH3 domain of FAD49.To test whether the PX domain could interact with anSH3 domain in FAD49, ... PXdomain to phosphoinositides might be regulated by the interaction between the PX domain and the third SH3domain, as in p47phox, although whether the interaction of the PX domain with the third ... lipid-binding activity of the PX domain in the resting state; phosphorylation of p47phoxreleases the binding, resulting in the active state,i.e. open conformation [25,35,36].As FAD49 contains...

Báo cáo khoa học: Heavy metal ions inhibit molybdoenzyme activity by binding to the dithiolene moiety of molybdopterin in Escherichia coli pdf

... with the attachment of GMP to the phosphate group of Moco, forming the MPT guaninedinucleotide cofactor (MGD) [14,15]. In total, morethan 10 genes are involved in the biosynthesis of Moco in ... caused by bind-ing to zinc binding proteins, e.g. proteins that containzinc ﬁnger protein structures [30]. Zinc and cadmiumare in the same group in the Periodic Table, contain the same common ... intestinal wall in humans with relative ease [34–36]. Vanadate ions also mimicmost of the rapid actions of insulin in the cell [37]. In this report, we examined the in vivo and in vitroincorporation...

Báo cáo khoa học: On peptide bond formation, translocation, nascent protein progression and the regulatory properties of ribosomes ppt

... Mutations in the 23S ribosomal RNA in the vicinity of the double hookbinding or in the b-hairpin of protein L22 were shown tobypass the elongation arrest [72]. The swing of protein L22 hairpin tip ... space of the growing chain. Consequently, the motions of the entire chain required for progression of the nascent chain within the tunnel, should be minimized, and the collision between the tryptophane ... the N-terminus of the nascent chain has already emerged out of the exit tunnel, thus may interact with the ÔpullingproteinÕ. This suggestion is consistent with the ﬁnding that in SecM protein,...

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