... of ADP and Mg2+ and followed by nondenaturing PAGE, the bands of theenzyme and the reactivase were markedly reducedin density, and two bands of the enzymeÆreactivasecomplexes (upper and lower) ... c,e, and g). To determine their subunit compositions,bands i–vi were excised and subjected to SDS⁄ PAGEon 6% and 14% gels (Fig. 5B,C), followed by densito-metric analyses. Both band i and band ... aR and bRin bands i and vi were determined to beabout 2 : 2 : 2 : 2 : 1 and 1 : 1 : 1 : 2 : 1, respectively,by densitometric analysis. Therefore, it was demon-strated that bands i and vi...