... substrates by the Conuscarboxylase. The propeptide of these substrates binds tightlyto the carboxylase and, as with the bovine carboxylase,represents all or almost all of the binding energy for ... withMEGALIGNprogram), and 65% are conserved usingBLASTanalysiscomparing all of the vitamin K-dependent carboxylases. In contrast, the C-terminal 25% of the protein shows nohomology to any of the other vitamin ... substitution. Using the bovinenumbering system (Fig. 1), we observe Arg179 rather thanhistidine, Thr430 rather than alanine, Pro654 rather thanserine, Met726 rather than threonine, and Gly743 ratherthan...