Báo cáo y học: "MMP-3 expression and release by rheumatoid arthritis fibroblast-like synoviocytes induced with a bacterial ligand of integrin α5β" pdf
Báo cáo y học: "MMP-3 expression and release by rheumatoid arthritis fibroblast-like synoviocytes induced with a bacterial ligand of integrin α5β" pdf
... Yli-Jama T, Jal-ava J, Vainio O, Toivanen P: Bacterial components in the syno-vial tissue of patients with advanced rheumatoid arthritis orosteoarthritis: analysis with gas chromatography-mass ... (RA) and osteoarthritis (OA) fibroblast-like synoviocytes (FLSs)Gene expression patterns in rheumatoid arthritis (RA) and osteoarthritis (OA) fibroblast-like synoviocytes (FLSs). Total RNA of ... aggressivebehavior of RA FLSs by inducing the release of pro-inflammatorycytokines and a cartilage-degrading enzyme, such as IL-6 and MMP-3, respectively.Keywords: fibroblast-like synoviocytes, integrin...
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx
... promoterTGA AGC TGG AAA CCA TCA TTC AAA ACA TTA GCA GGA ATT TTC 52 343Lower promoter GGA GTT CTG CCA GGG AAC CAC GAC AGG GGA GAA CGC CAC TTA 57 587Exon 1 GTG CTG CCT GAG AAG GAT TG GAA AGT GCC ... exactly with the mobilities of di-, mono- and unglycosylated forms of recombinantcystatin F previously characterized by enzymatic removal of N-linked carbohydrate and carbohydrate compositionanalysis ... incubated with a specific polyclonal antiserum againstrecombinant cystatin F and subsequentlyhybridized with a secondary FITC labelledantibody against rabbit IgG. A vesicularstaining pattern can...
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Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii ppt
... cytoplasmic fraction (20 lg, lane1) and of CCT purified from rabbit reticulocyte lysate (2 lgand6lgin lanes 2 and 3, respectively). Lanes 1 and 2 were Western blotted and immunostained by an ... CCT were quantitated by the use of a phosphori-mager and expressed as a fraction of the maximum amount of boundlabeled tubulin and plotted as a function of the ratio of labeled : un-labeled tubulin.Ó ... mammals. Then, thepUC18 containing the mutated b-T1 was used as a templatein a PCR with the oligonucleotides 5¢-CGAGCTCGGTACATATGAGAG-3¢, as forward primer, and 5¢-TCGACTCTAGAGGATCCCC-3¢, as...
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Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot
... recombinant NaoAafter DEAE-Sepharose Fast Flow chromatography; lane 6, standardmolecular mass markers (phosphorylase b,97kDa;BSA,66kDa;ovalbumin, 45 kDa).Fig. 3. Assay of NaoA activity. (A) Protein ... NaoA was determinedfrom a standard curve of pI and migration distance (cm) of protein standards.Enzyme assay and analytical methodsE. coli BL21(DE3) containing plasmid pNA101 was grownat 37 ... stronginhibitors of NaoA, and thiol compounds and superoxidedismutase partially inhibit the enzyme activity. Therefore,superoxide may be an essential intermediate in the oxidation of nitroalkane by NaoA.Keywords:...
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Báo cáo y học: "Gene expression profiling in murine autoimmune arthritis during the initiation and progression of joint inflammation" pps
... This paw was designated as acutearthritic (AA), and contralateral or ipsilateral paws that were Arthritis Research & Therapy Vol 7 No 2 Adarichev et al.R199 with cartilage PG, and live lymphocytes ... course of the adoptively transferred arthritis, RNA was isolatedfrom pre-arthritic paws (PA) and diseased paws (AA and CA) (Table 1). In addition, RNA was isolated from normalpaws of naive BALB/cSCID ... normal paws of naive BALB/cSCID mice (AN), clinically normal pre-arthritic paws (PA), acutely arthritic paws (AA) and chronically inflamed paws (CA).Available online http:/ /arthritis- research.com/content/7/2/R196R200expressed...
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Báo cáo y học: "Gene expression and activity of cartilage degrading glycosidases in human rheumatoid arthritis and osteoarthritis synovial fibroblast" potx
... (tetrasaccharides and hexasaccharides of hyaluronate and heparan sulphate) [39-41]. Interestingly, allknown carbohydrate TLR ligands fall into the category of oli-gosaccharides generated by endoglycosidases, ... in arthritis patients' syno-vial membrane and fibroblast samplesHc-gp 39, HexA and HexB gene expression in arthritis patients' syno-vial membrane and fibroblast samples. Rheumatoid arthritis ... present data, glycosidasesexpressed by synovial membranes and synovial fibroblasts areunder negative regulation by some locally expressed cytokinesboth in rheumatoid arthritis and osteoarthritis....
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Báo cáo y học: "Gene expression profiles in the rat streptococcal cell wall-induced arthritis model identified using microarray analysis" docx
... 5'-CACAGTGAGGAATGTCCACAAAC 5'-6-FAM-TCGGCAAACCTAGTGTGCTATGCCTAAGCA-TAMRATNF-α 5'-CCAGGTTCTCTTCAAGGGACAA 5'-CTCCTGGTATGAAATGGCAAATC 5'-6-FAM-CCCGACTATGTGCTCCTCACCCACA-TAMRAGRO1 ... 5'-6-FAM-TTCTATGCGCGGGGGCGGAA-TAMRACD3 5'-GGATGGAGTTCGCCAGTCAA 5'-GGTTTCCTTGGAGACGGCTG 5'-6-FAM-ACAGGTCTACCAGCCCCTCAAGGACCG-TAMRAUbiquitin 5'-CGAGAACGTGAAGGCCAAGA 5'-GGAGGACAAGGTGCAGGGTT 5'-6-FAM-CCCCTGACCAGCAGAGGCTCATCTTTG-TAMRAIL, ... 5'-TGTGTTGAAGCTTCCCTTGGA 5'-TGAGACGAGAAGGAGCATTGGT 5'-6-FAM-TGTCTAGTTTGTAGGGCACAATGCCCT-TAMRACD14 5'-GGACGAGGAAAGTGTCCGCT 5'-AGGTACTCCAGGCTGCGACC 5'-6-FAM-TTCTATGCGCGGGGGCGGAA-TAMRACD3...
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Báo cáo y học: "Aerobic exercise and its impact on musculoskeletal pain in older adults: a 14 year prospective, longitudinal study" doc
... scores by age, gender and study groupAdjusted mean visual analog scale pain scores by age, gender and study group. Significant increases in pain scores for female and male community controls and ... pro-duce covariate-adjusted mean VAS pain by exercise group and by study year for statistically significant predictors that y yytttt=+∈−12,Available online http:/ /arthritis- research.com/content/7/6/R1263R1267female ... performed statistical analyses, interpretation of data, and drafting of the manuscript; JFF participated in study design,interpretation of data, and drafting of the manuscript; DL par-ticipated in...
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Báo cáo y học: "PTPN22 polymorphism and anti-cyclic citrullinated peptide antibodies in combination strongly predicts future onset of rheumatoid arthritis and has a specificity of 100% for the disease" ppsx
... combination of the T variant and HLA-SE (OR = 7.85). The PTPN22 1858T variant was associated with future development of RA. There was an associationbetween the T variant and anti-CCP antibodies and ... wassignificantly associated with carriage of the T variant and therewas a greatly increased relative risk for the development of RAAvailable online http:/ /arthritis- research.com/content/8/1/R19Page 5 of ... combination of anti-CCP antibodies and carriage of the PTPN22 1858T vari-ant gave a very high OR compared with not having any of them. As none of the controls had this combination, the ORwas calculated...
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Báo cáo y học: " Environmental risk factors differ between rheumatoid arthritis with and without auto-antibodies against cyclic citrullinated peptides" potx
... frequency-matched by gender and birthyear were randomly selected from the Danish population by means of the Civil Registration System, a national databasethat keeps track of all demographic changes ... birth year, and year or pseudo-year of RA diagnosis. bAdjusted for gender, birth year, year or pseudo-year of RA diagnosis, place of residence, educational status, history of urinary tract infection, ... non-significantly associated with RA risk. Physician-verified asthma before age 45 years and a history of urinarytract infection were both associated with significantly reducedrisk of RA. No statistically...
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