... identical in the single and double mutants involving this residue (Fig. 5B,D). In both cases, the side chain is removed from the vicinityof Ser278 and Asp82 and the catalytic triad does notexist in ... estimated from percentageintensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and 19-kDa bands, where the intensity of the38-kDa protein band was corrected for ... was initiallyknown as pepstatin-insensitive acid peptidases [2,3].However, recent crystallographic and modeling studiesrevealed that the sedolisins (sedolisin, kumamolisin,kumamolisin-As, and...