Báo cáo khoa học: "A colliding maxillary sinus cancer of adenosquamous carcinoma and small cell neuroendocrine carcinoma - a case report with EGFR copy number analysis" pot

Báo cáo khoa học: Thyroid hormone induces the expression of 4-1BB and activation of caspases in a thyroid hormone receptor-dependent manner pptx

... TRAF1 were designated HeLaTR/ 4-1 BB and HeaLaTR/TRAF1, respectively. Primers for amplifying the 4-1 BB and TRAF1 cDNAs were: 5¢-GAATTCAAGCTTATGGGAAACAGCTGTTACAACATA-3¢ and 5¢-GAATTCAAGCTTCACAGTTCACATCCTCCTTCTTCT-3¢ ... 5¢-GAATTCAAGCTTCACAGTTCACATCCTCCTTCTTCT-3¢ for 4-1 BB,5¢-CCCGGGATATCATGGCCTCCAGCTCAGGCAGCAGTC-3¢ and 5¢-CCCGGGATATCTAAGTGCTGGTCTCCACAATGCACT-3¢ for TRAF1.Isolation of RNA and RT-PCRSub-conﬂuent cells in 10-cm ... of the primers used for amplifying the hTRa1cDNA were: 5¢-CCCGGGAAGCTTCGGACCATGGAACAGAAGCCAAGCAAGGTG-3¢ and 5¢-CCCGGGGTCGACGACTTCCTGATCCTCAAAGACCTC-3¢.Inorder to overexpress 4-1 BB and TRAF1...

Báo cáo khoa học: Computer-assisted mass spectrometric analysis of naturally occurring and artiﬁcially introduced cross-links in proteins and protein complexes potx

... of chemical cleavage at aspartate residues. We wereaware of the possible occurrence of deamidations of asparagines and subsequent partial cleavage at theresulting aspartate residues. However, virtualmslabanalysis ... moderate number of alternativecandidates should still allow unambiguous identiﬁca-tion based on additional tandem mass spectrometricvalidation. It thus appears that a cross-linkingapproach ... data obtained from the equivalent real experi-ments. Matches between virtual and acquired data reveal the identity and nature of reaction products that may lead to characterization of post-trans-lational...

Báo cáo khoa học: Frataxin deﬁciency causes upregulation of mitochondrial Lon and ClpP proteases and severe loss of mitochondrial Fe–S proteins pot

... Sciences, Meylan, France).Lon protease (forward, 5¢-AGGATCTTGCCTTGTGTGGA-3¢; reverse, 5¢-TGGATGAGGAGCTGAGCAAG-3¢)ClpP (forward, 5¢-CACACCAAGCAGAGCCTACA-3¢;reverse, 5¢-CCCAGCAGAGGAAGTTTCAG-3¢), mitochon-drial ... 5¢-GGAGGGCAAGCAACAGTATC-3¢; reverse,5¢-GCGTTCCTCTGTGAAGTCGT-3¢), Rieske (forward,5¢-TGGTCTCCCAGTTTGTTTCC-3¢; reverse, 5¢-GCAGCTTCCTGGTCAATCTC-3¢) and SDHA (forward, 5¢-CAGAAGTCGATGCAGAACCA-3¢; ... mitochon-drial aconitase (forward, 5¢-AGGAGTTTGGCCCTGTACCT-3¢; reverse, 5¢-GCCTTGAATGGTCAGCTTGT-3¢),NDUFS3 (forward, 5¢-CTGTGGCAGCACGTAAGAAG-3¢;reverse, 5¢-ACTCATCAAGGCAGGACACC-3¢), SDHB(forward,...

Tài liệu Báo cáo khoa học: Differentiation stage-dependent preferred uptake of basolateral (systemic) glutamine into Caco-2 cells results in its accumulation in proteins with a role in cell–cell interaction pptx

... 5.2 ± 0.3 for 5-day- and 15-day-differentiated cells, respectively. At 24 hthe basolateral ⁄ apical uptake ratio was 3.0 ± 0.6 and 1.7 ± 0.3 for 5-day and 15-day-differentiated cells,respectively. ... functional groups. Theﬁrst group of proteins (annexin A2 , annexin A4 , cad-herin-17, galectin-3 and alpha-actinin 4) is involved inmembrane stabilization, cell cell adhesion and cell matrix adhesion, ... dose and route of supplementation. Data from a meta-analysissuggested that glutamine supplementation in criticallyill patients may be associated with a decrease in com-plications and mortality...

Tài liệu Báo cáo khoa học: Oxygen tension regulates the expression of a group of procollagen hydroxylases docx

... ISense:5¢-CGGGATCCTCGGACACCCTGTAAATG-3¢Antisense:5¢-GGAATTCCAAGCAGTCCTCAGCTGT-3¢Mouse (gi:6754969) prolylhydroxylase alpha IISense:5¢-CGGGATCCTGCAGGCAGAATTCTTCA-3¢Antisense:5¢-GGAATTCCCAGTCTGTGTTCAACCG-3¢Rat ... lysylhydroxylases mRNAs.Mouse (gi:836897) prolylhydroxylase alpha ISense:5¢-CGGGATCCTAGACCGGCTAACAAGTA-3¢Antisense:5¢-GGAATTCCAAGCAGTCCTCAGCTGT-3¢Rat (gi:474939) prolylhydroxylase alpha ISense:5¢-CGGGATCCTCGGACACCCTGTAAATG-3¢Antisense:5¢-GGAATTCCAAGCAGTCCTCAGCTGT-3¢Mouse ... Biosciences) and ana-lyzed with theIMAGE3.1 analysis software package (Amer-sham Bioscience). Each spot was matched from one gel toanother and the relative volume of matched spots wascompared....

Báo cáo khoa học: Post-ischemic brain damage: targeting PARP-1 within the ischemic neurovascular units as a realistic avenue to stroke treatment pptx

... triggersDNA damage-dependent hyperactivation of PARP-1,depletion of intracellular NAD and ATP stores, and neuronal death [26]. PARP-1 activation may also occurin neurons without NMDA receptor activation, ... result of the fact thatinﬂammatory transcription factors such as nuclearfactor-kappaB, activator protein-1 and nuclear factor of activated T-cells are positively regulated byPARP-1. PARP-1 protein ... complex cardiovascular and cerebral pathophysiology of stroke patients, and neuroprotection should be evaluated on a long-lasting and functional basis, rather than on an acute and his-tological basis....

Báo cáo khoa học: Epl1, the major secreted protein of Hypocrea atroviridis on glucose, is a member of a strongly conserved protein family comprising plant defense response elicitors potx

... [e.g.cerato-platanin of Ceratocystis ﬁmbriata f. sp. platani,Snodprot1 of Phaeosphaeria nodorum and Sp1 of Leptosphaeria maculans) or human allergens and path-ogenesis-related proteins (As-CG of Coccidioides ... detected, and byfar the most abundant spot (g1 in Fig. 1) was a small protein (approximately 16 kDa, pI 5.5–5.7), and thiswas followed by spot g2, with a similar pI but a with a molecular mass of approximately ... phase, standard bed; Millipore) [47]. Samplepreparation for MALDI MS was carried out on a stainlesssteel target, using a thin-layer preparation technique [48] with a- cyano-4-hydroxy-cinnamic...

Báo cáo khoa học: The natural mutation by deletion of Lys9 in the thrombin A-chain affects the pKa value of catalytic residues, the overall enzyme’s stability and conformational transitions linked to Na+ binding pdf

... thrombin, the A- chain assumes an overallboomerang-like shape interacting with the B-chain sur-face opposite to the active site [17]. Stabilization withinthe A- chain and between the A- and B-chains ... report, DK9 compared with WT thrombin achieves a better interaction with thelow-molecular mass inhibitor N -a- (2-naphthylsulfonyl-glycyl )-4 -amidinophenylalanine-piperidine (a- NAPAP)[2,8]. As ... (2–4). Global and simul-taneous analysis of the experimental data by grafit soft-ware allowed computation of the pK a values of the twogroups both in the free and substrate-bound species of thrombin...

Báo cáo khoa học: Evidence for two different electron transfer pathways in the same enzyme, nitrate reductase A from Escherichia coli potx

... high-potential and a low-potential class. The high-potential classcontains [4Fe-4S] and [3Fe-4S] clusters with redox potential of +180 mV and +130 mV, respectively. Redox potentials of )55 and )420 mV ... time constantscorresponding to apparent rate constants are obtained byﬁtting Eqn (1):DAbs ¼ a þ b eÀctð1Þwhere, DAbs is the variation of absorbance, a and b areamplitude parameters and c is ... side, and Fig. 5. Spectra of membranous nitrate reduc-tase cytochrome of mutant devoid of the high-est-potential [4Fe-4S] cluster. Reduced spectraare shown as solid lines and oxidized spectraas...

Báo cáo khoa học: Unravelling the functional interaction structure of a cellular network from temporal slope information of experimental data docx

... cerevisiaeMAP kinase cascades typically composed of three tiers of protein kinases, a MAP kinase (MAPK), a MAPK kinase(MAPKK) and a MAPKK kinase (MAPKKK), arecommon signalling modules in eukaryotic cells ... that can beapplicable to time-series experimental data withoutparameter perturbations and that can make use only of the qualitative information without relying on meas-ured absolute values. ... 1760–1763.23 Maeda T, Wurgler-Murphy SM & Saito H (1994) A two-component system that regulates an osmosensingMAP kinase cascade in yeast. Nature 369, 242–245.24 Maeda T, Takekawa M & Saito H...

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