... USA). All other chemicals, obtained locally from Merck (India), were ofanalytical grade.For all experiments, the protein concentration is reportedas the concentration of monomeric CII. Unless otherwisestated, ... Effect of salt on CII. Molar ellipticity (at 220 nm) and ANSfluorescence (kex¼ 360 nm; kem¼ 495nm)ofCIIprotein(9lM)inpresence of GdnHCl, other salts and urea. Values shown were nor-malized ... of ANS fluorescence with increasing GdnHCl. ANS wasadded to the protein solution (incubated in presence of GdnHCl for 30 min) to a final concentration of 15 lM, and the fluorescence emis-sion...