... analysis by the amplification of housekeeping gene GAPDH as describedpreviously [25]. Mouse TPH was amplified by a single PCR,while serotonin AANAT was amplified by nested PCR. The localization of ... Memphis, TN, USA;4Department of Internal Medicine, Southern Illinois University, Springfield, IL, USAWe showed expression of the tryptophan hydroxylase geneand of tryptophan hydroxylase protein ... all samples of mouse corporal skin, although it was detectable at low levels in the ears and in Cloudman melanoma (derived from the DBA/2 J mouse strain). In conclusion, mouse skin has the molecular...