Báo cáo khoa học: "Physiological correlations and bud dormancy in the apple tree (Malus domestica Borkh )" pdf

Báo cáo khoa học: "Physiological correlations and bud dormancy in the apple tree (Malus domestica Borkh.)" pdf

... treatments. The inhibited state of the lateral buds in apple could be responsible for the shallow dormancy exhibited by these buds. Never-theless, the potential for these buds ... dor-mancy in the lateral buds can be consid-ered a good means to study, using struc-tural and biochemical approaches, the mechanisms involved in bud dormancy in apple trees.AcknowledgmentsSpecial ... October modified the course of bud dormancy (i.e., before the peak MTB in late November). The intensity of dormancy in the uppermost buds was considerablyincreased for treated...

Báo cáo khoa học: Physiological truncation and domain organization of a novel uracil-DNA-degrading factor pdf

... characterize the potential involvement of the C-terminal region of UDE in DNA binding, Asp-Nendoproteinase was also used for limited proteolysis,as the C-terminus of the protein is rather rich in Aspresidues ... Germany), usingNdeI and XhoI sites. The recombinant construct included aHis6tag and a linker segment at the C-terminus.Western blottingWestern blotting was performed as described in [15], usinganti-DmUDE ... signiﬁcantprotection against proteolytic cleavage along motifs1A and 1B, indicating either DNA-binding-inducedconformational changes or covering of otherwiseexposed proteolytic sites by DNA binding to thesesegments.Motif...

Tài liệu Báo cáo khoa học: Membrane targeting and pore formation by the type III secretion system translocon pdf

... assembly. In the ﬁrst model, both hydrophobic translocators exist in oligomeric form, with the major partner insertedstably into the membrane, whereas the minor protein is the link with the V antigen. ... isnot the case for its counterparts in other bacteria. Itsinteraction with macrophages induces a decrease in the production of the pro -in ammatory cytokines tumournecrosis factor-a and interferon ... that the needle tip, which in principle contacts all three translocon components,exists in ‘on’ and ‘off’ states [52], thus suggesting thatall proteins involved in the initial contact with the target...

Báo cáo khoa học: Prevalent conformations and subunit exchange in the biologically active apoptin protein multimer potx

... measured. We determined the amount of MBP–apoptin-FM incorporated intoMBP–apoptin-H6by measuring the ratio of FM vs. Trpﬂuorescence in the eluted protein. We found that the histidine-tagged multimers ... multimerdespite the presence in MBP–apoptin of a large, highlysoluble protein tethered to the N-terminus of apoptin.Secondly, the dye-binding behaviour of MBP–apoptinsuggested that certain conformers ... cross-linking it with glutaraldehyde [2].We found that the increase in FFM/FIAwith cross-linkedMBP–apoptin-FM amounted to % 15% of the effect weobserved with noncross-linked protein, indicating...

Báo cáo khoa học: "Drought susceptibility and xylem dysfunction in seedlings of 4 European oak species" pdf

... delay or mishandling.Losses could then be considerable due toxylem dysfunction not only in the leaflessstem but in the few roots that remain after the seedling has been ... 1990). An increased inci-dence in eastern Europe in the 1980s hasbeen of concern following the 1976 drought,affecting Quercus robur in particular.Although there is no ... mid-Euro-pean, and are widespread in the UnitedKingdom, while the other 2 are more xeric,drought resistant species, commonly found in southern Europe and therefore providea...

Báo cáo khoa hoc:"Genetic parameters and genetic trends in the Chinese x European Tiameslan composite pig line. I. Genetic parameters" docx

... 19] and the USA [42] conﬁrmed the high proliﬁcacy, the good moth-ering ability and the strong hardiness of Meishan, Jiaxing, Fengjing and Minpurebred and crossbred sows. Their poor growth and ... of a Chinese × European pig line. I 43multiparous sows from the Laconie line. The Laconie line was constructed in 1973 and has been maintained as a closed line and selected for growth and carcass ... production and reproduction traits in the Tiameslan line.2. MATERIAL AND METHODSCreation, management and selection of the Tiameslan lineTwo similar sublines were created in 1983 and 1985 in the nucleus...

Báo cáo khoa hoc:" Genetic parameters and genetic trends in the Chinese x European Tiameslan composite pig line. II. Genetic trends" pdf

... creating a Chinese × European compositeline and selecting it for growth and carcass traits [1]. The Chinese × EuropeanTiameslan line was created in 1983 by the Pen Ar Lan breeding company and has ... selection during the last few years. However, the selection for whitecoat color and the eradication of the unfavourable allele at the RN locus [19]conducted since the beginning of the nineties are ... NW and SURV included parity and farrowing batch as fixed effects, the additive genetic value and the permanentenvironment of the sow as random effects, as well as age within parity and sow and/ or...

Báo cáo khoa học: " Uncoupling GP1 and GP2 expression in the Lassa virus glycoprotein complex: implications for GP1 ectodomain shedding" ppt

... deleted, the intracellular domain (IC) was fused to the ectodomain, and the gene wasco-expressed with a complete GP1 gene in cis; (2) the TM and IC domains were deleted and GP1 was co-expressed in cis; ... RRLL results in the N-ter-minal 42-kDa glycoprotein 1 (GP1) subunit and the C-terminal 38-kDa glycoprotein 2 (GP2) subunit containinga transmembrane (TM) and intracellular (IC) domain [8].GP1 ... [30], and all recom-binant plasmids were engineered and propagated in Escherichia coli (E. coli) strain DH5α, according to the manufacturer's instructions (Invitrogen). The DNAsequence and...

Báo cáo khoa học: "Elevated troponin and myocardial infarction in the intensive care unit: a prospective study" potx

... screening of all patients with troponin and ECGrecordings was not performed in this study, and hence wecannot determine the true prevalence and incidence of ele-vated troponin and MI. Finally, there ... should determine the prognostic importance of ele-vated troponin in the ICU by more comprehensively examiningother diagnoses and their consequences, and by evaluating the role of other modalities ... notbeen studied. In view of the prognostic and possible therapeu-tic implications of establishing a diagnosis of MI in the criticallyill, use of noninvasive tests – including troponin and ECG –may...

Báo cáo khoa học: " Mechanical ventilation and lung infection in the genesis of air-space enlargement" pptx

... old, weight 20 ± 2 kg) were anesthetizedusing propofol (3 mg/kg) and orotracheally intubated in the supine position. Anesthesia was maintained with a continuousinfusion of midazolam (0.3 mg/kg ... pancuronium (0.3mg/kg per hour), and fentanyl (5 μg/kg per hour). A catheterwas inserted in the ear vein for continuous infusion of 10%dextrose and Ringer lactate, and the femoral artery was cannu-lated ... non-ventilated-inoculated and ventilated-inoculated piglets lying in the prone position. Fortymilliliters was instilled in each lower lobe and 10 ml in eachmiddle lobe.Fixation of the lungs The piglets...

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