... with the Biorad Protein Assay Kit (Biorad,Hercules, CA, USA) and BSA as a standard.Determination of the equilibrium constantsby ELISA The dissociation constants at equilibrium in solution, ... towards this virus to the exclusion of other flavi-viruses, the cross-reactivities towards the four viralserotypes, and the mechanisms of neutralization at a molecular level. The diversity of the ... antiparallelb-strands can give rise to a dipolar interaction (nOe). A comparison of the NOESY spectra of MalE-E3-H6and MalE allowed us to unambiguously assign fourinterstrand H a -H a nOe signals to the E3...