... acid epoxides, are also metabolized by EHs. Thereare five classes of EH in mammals: soluble (sEH), micro-somal (mEH), hepoxilin A3hydrolase, leukotriene A4hydrolase, and cholesterol 5,6-oxide ... 305–340.2.Oesch,F.,Herrero,M.E.,Hengstler,J.G.,Lohmann,M.&Arand, M. (2 000) Metabolic detoxification: implications forthresholds. Toxicol. Pathol. 28, 382–387.3. Fretland, A.J. & Omiecinski, C.J. (2 000) Epoxide hydrolases:biochemistry ... saline containing 2 mMEDTA. Thecell pellets were suspended directly in two volumes ofsample-treatment buffer (1 0% urea, 2.5% SDS, 5%2-mercaptoethanol, 0.005% bromophenol blue) then boiledfor...