báo cáo khoa học: "The management of advanced oral cancer in a Jehovah''''''''s Witness using the Ultracision Harmonic Scalpel" docx

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... humans, the ﬁrst characterization of IVD deﬁciency in metazoa.Considering that IVD acts in the third step of leucine degradation and the sku mutant accumulates branched-chain amino acids in haemolymph, ... biochem-ical data, along with previous observations that the sku mutant accumulates isovaleric acid and branched-chain amino acids, strongly indicate that a singleamino acid substitution (G376V) in ... accumu-lations of branched-chain amino acids, includingleucine, in haemolymph [6]. From the second feature,it can be assumed that the branched-chain amino aciddegradation mechanism might be dysfunctional...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... N,Kamogawa J, Yamamoto H, Takubo N, Nakata S,Yamada K, Yamamoto S et al. (2005) Accumulation of plasma cells expressing CXCR3 in the synovial sublin-ing regions of early rheumatoid arthritis in associationwith ... RM(1994) Macrophage in ammatory protein-1 alpha. A novel chemotactic cytokine for macrophages in rheuma-toid arthritis. J Clin Invest 93, 921–928.25 Hatano Y, Kasama T, Iwabuchi H, Hanaoka R,Takeuchi ... 14 days led to severe arthritischaracterized by apparent erythema and joint pain,accumulation of leucocytes, in ltration of mononuclearcells in synovial tissue and marked hypervascular-ization...

Tài liệu Báo cáo khoa học: Different modes of dipeptidyl peptidase IV (CD26) inhibition by oligopeptides derived from the N-terminus of HIV-1 Tat indicate at least two inhibitor binding sites doc

... C-terminalGlu9 of Tat(1–9) and the side chains of Arg560 and Lys554 of DP IV. Furthermore, the side chain of Asp5 of Tat(1–9) wasalso able to interact with the side chain of Lys554 of DP IV.Additionally, ... hydrophobicinteractionofthesidechainofMet1ofTat(1–9)withthephenyl ring of the substrate Ala-Pro-pNA resulted in a ﬁxation of the aromatic leaving group.Rather similar interactions were obtained for docking of Trp2-Tat(1–9) to DP IV in the presence of the ... between the backbone carbonyl group of Val4 of the peptide and the backbone amide hydrogen of Ala743 of the enzyme, between the side chain carbonyl group of Asn7 of Tat(1–9) and the side chain of...

Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf

... have an aspartate as the catalytic apparatus.However, for chymotrypsin-type serine proteases, the replacement of this aspartate with an alanine diminishesprotease activity 104-fold [24]. Therefore, ... & Petsko, G .A. (1986) Amino–aromatic interactions in proteins. FEBS Lett. 203, 139–143.19. Chakrabarti, P. & Samanta, U. (1995) CH/, p interaction in the packing of the adenine ring in ... typically W169V andW16 9A, deviates from the original position. In the struc-tural deviation, the solvent ASA of the side-chain of His210increased with the decrease in size of the side-chain atresidue...

Báo cáo khoa học: Heterologous synthesis of cytochrome c¢ by Escherichia coli is not dependent on the System I cytochrome c biogenesis machinery ppt

... (1996) An in vivo pathway for disulfide bond isomerization. ProcNatl Acad Sci USA 93, 13048–13053.28 Oikawa K, Nakamura S, Sonoyama T, Ohshima A, Kobayashi Y, Takayama SJ, Yamamoto Y, UchiyamaS, ... Judging from the staining intensity, the level of production of the PHCP protein in the presence of the pEC86 plasmid was significantly lower than thatwithout the plasmid, indicating that the ... JapanIntroductionCytochromes c¢ are classified as class II cytochromes caccording to Ambler [1], and are found in the peri-plasm of certain Gram-negative Alpha-, Beta- andGammaproteobacteria. Recent biochemical and geneticanalyses...

Báo cáo khoa học: Identiﬁcation of tyrosine-phosphorylation sites in the nuclear membrane protein emerin pot

... nuclear membrane (INM). The ONM is part of the rough endoplasmic reticulum and folds at the nuclear pores into the INM, which is ﬁrmly attachedto the lamina by integral membrane proteins of the INM. ... based on in vivolabeling of all the cellular proteins by isotope-codedamino acids. In addition, we used the determination of relative ratios of peptide abundance obtained fromproteins isolated ... both approaches by already adding a cell-permeable tyrosine-phosphatase inhibitor based onpervanadate before opening up the cells. The compar-ison of the tyrosine-phosphorylation status of nuclearenvelopes...

Báo cáo khoa học: Membrane binding of SRP pathway components in the halophilic archaea Haloferax volcanii docx

... interaction of SRP pathway components in Archaea, the membranebinding of SRP54 and FtsY in the halophilic archaeaHaloferax volcanii was investigated. Such studies offerinsight not only into the ... positive charges at the start of the A domain of haloarchaeal FtsY proteins, it is notunreasonable to implicate an additional portion of the protein in membrane binding. In E. coli, it has also beenreported ... SRP54binding, a substantial amount of SRP54 can bind to SRPRNA in the absence of SRP19 in Archaea [18–20]. Morever, the precise role of archaeal SRP in protein translocationremains an open...

Báo cáo khoa học: Thermal behaviour of cubic phases rich in 1-monooleoyl-rac-glycerol in the ternary system docx

... getsthinner, with the minimal and the parallel surfaces of the polar/nonpolar interface approaching each other, againresulting in an increased wedge shape of the lipids.Previously, Ai and Caffrey ... Diﬀractogram of sample A showing (A) without and (B) with the extra peak. The dots above the peaks indicate the calculated positionsobtained for the Pn3m structurebasedontheﬁrstpeakinthedif-fractogram.Table ... observation that upon addition of OG an increase in the size of the unit cell within a one-phase area arises, and from the order of occurrence of the phases formed. The reason for this change in the...

Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc

... until the unspecific rate of the background decline was again reached (Fig. 2A) . Subsequent addition of external NADH to the assayresulted in further reduction of the flavin-specific absorb-ance at ... withoutshaking. Anaerobic samples were taken for monitoring of growth at 600 nm and b-galactosidase activity determined. In E. coli strains carrying a plasmid encoding NifL andNifA (pNH3) or NifA alone ... in which no significant decrease of the NifLabsorbance at 450 nm was observed after the addition of NADH. Inside-out vesicles containing the anaerobicrespiratory chain were prepared from anaerobic...

Báo cáo khoa học: Proteolytic degradation of nitric oxide synthase isoforms by calpain is modulated by the expression levels of HSP90 potx

... PAGE and stained with the appro-priate antibody. The bands were then scanned and the area of the peaks obtained was used to create a calibrationcurve.Degradation of NOS isozymes by calpain ... close to the CaM binding site. This explains why both NOSare inactivated by calpain in the absence of HSP90. The removal of the PDZ domain by calpain, whichoccurs without loss of catalytic activity ... monoclonal anti-HSP90, nNOS and eNOS sera.Assay of calpain activityCalpain activity was assayed as previously described [39].One unit was deﬁned as the amount of enzyme causing the release from the...

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