... (DDDDK)was included within the forward primer immediatelyupstream of the start codon (ATG). Primers with the fol-lowing sequences were synthesized by Proligo (Paris,France): 5forGulox (forward), 5¢-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGATGACGACGACAAGATGAGCCCGATATGGAGTAATTGGCCT-3¢; ... l-gulono-1,4-lactone dehydrogenase of M. tuberculosis is a specific enzyme for the biosynthesis of L-AA. As far as we know, this is the first report of aspecific biosynthetic enzyme for vitamin C in bacteria.To ... mutants of the gene could be obtained[40]. The gene is well conserved within the M. tuber-culosis complex, except for the M. bovis BCG Pasteur(1173P2) strain, which lost the gene due to the dele-tion...