Báo cáo y học: "Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers" docx
Báo cáo y học: "Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers" docx
... sorted cellsReanalysis of triple positive sorted cells. (a) Forward and side scatter characteristics of sorted osteoarthritic cartilage cells. (b-d) CD9-fluorescein isothiocyanate (FITC)/CD166-phycoerythrin ... split by trypsintreatment (0.05% trypsin, 0.02% EDTA; Biochrom) at 75%confluence.Flow cytometry analysis of cells Either isolated cells from OC were directly used for flowcytometric analysis ... specific staining. The distribution of OC cells in forward/side scatter exhib-ited no difference between isotype and antibody staining. Amaximum of 2% positive cells by staining with isotype anti-body...
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Báo cáo y học: "Upregulated miR-146a expression in peripheral blood mononuclear cells from rheumatoid arthritis patients" doc
... prednisone, mycophenolate mofetilSjS1 Female 30 Hydroxychloroquine, mycophenolate mofetilSjS2 Male 21 HydroxychloroquineSSc1 Female 57 Cyclosporine, hydroxychloroquine, prednisone, mycophenolate mofetilaSample ... biomarkers for miRNA activity (Pauley KM, unpublisheddata), we began to examine a variety of cytokines and chemok-ines for their ability to stimulate miRNA activity in human mono-cytic THP-1 cells. ... at 37°C for 1 hour. Cells were fixed in 3% paraformaldehyde for 10 min-utes and permeabilized in 0.5% Triton X-100 for 5 minutes.GWB were detected in THP-1 cells with a human prototypeanti-GWB...
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Báo cáo y học: "Identification of clinical and simple laboratory variables predicting responsible gastrointestinal lesions in patients with iron deficiency anemia"
... Guidelines for the man-agement of iron deficiency anemia for the British Society of Gastroenterology. Gut. 2000; 46: 1–5. 28. Rockey DC, Cello JP. Evaluation of gastrointestinal tract in patients with ... evalua-tion of patients with IDA may delay the diagnosis of GI tumors especially colorectal cancer [12]. In this study, we aimed to evaluate the diagnos-tic yield of endoscopy in patients with IDA ... diagnosed adenocarcinoma by endoscopic biopsy. A right hemicolectomy was performed, and the patient had no any metastasis. Three years after surgery, he is alive without any symptoms. The standard...
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Báo cáo y học: "Identification of Cellular Membrane Proteins Interacting with Hepatitis B Surface Antigen using Yeast Split-Ubiquitin System"
... Split-Ubiquitin Yeast Two Hybrid Screening 2.3.1 Mating of S. cerevisiae DSY-1 (Bait) and S. cerevisiae DSY-2 (Prey) The 1ml aliquot (≥2x107 cells) frozen cDNA library was added to 5ml bait cells ... in-frame with Cub (the yeast ubiquitin). The construct was then used to transform yeast S. cerevisiae DSY-1 strain by LiOAc method. 2.2 Construction of cDNA Library from HBV-transfected HepG2 Cells ... system is not suitable for the analysis of interaction between membrane bound proteins. The split-ubiquitin yeast two hybrid system has been recently developed for the characterization of...
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Báo cáo y học: "Identification of a SmD3 epitope with a single symmetrical dimethylation of an arginine residue as a specific target of a subpopulation of anti-Sm antibodies" ppsx
... Germany2Professor of Medicine, Faculty of Medicine, University of Calgary, Calgary, Canada3Vice Director of Autoimmune Diagnostics, Laboratory of Prof. Seelig and colleagues, Karlsruhe, GermanyCorresponding ... Germany), Prof.Dr MJ Fritzler (University of Calgary, Calgary, Canada) andby Labor Limbach (Heidelberg, Germany).To assess further the assay specificity, we analyzed agroup of sera from patients ... absence of inhibition can beexplained by the variety of different anti-Sm antibody sub-populations and by the variety of corresponding epitopes.Recently, two polyclonal antibodies (SYM10, SYM11)were...
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Báo cáo y học: " Identification of functional modules that correlate with phenotypic difference: the influence of network topology" potx
... implicated in ba sal cell carcinoma inmice [50]; more generally, abnormal activity of hedge-hog-GLI is associated with a variety of tumor types [51].The coexistence of basal cell carcinoma and ... Immunobiology of endometriosis. FertilSteril 2001, 75:1-10.57. Kao LC, Germeyer A, Tulac S, Lobo S, Yang JP, Taylor RN, Osteen K,Lessey BA, Giudice LC: Expression profiling of endometrium from women with ... expression profiles of two p henotypes, a list of gene sets, and their topology.Inthisstudy,thegenesetsaretakenfromtheKEGGdatabase [64] as of April 2009: the gene files specifygenes in a pathway and...
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Báo cáo y học: "Special considerations in the treatment of patients with bipolar disorder and medical co-morbidities"
... recommended before initiating treatment with antipsychotics. However, a careful medical history shouldbe taken for symptoms of cardiac pathology, such asrecurrent syncope. A family history of early sudden ... year of therapyAnnals of General Hospital Psychiatry 2004, 3 http://www.general-hospital-psychiatry.com/content/3/1/7Page 5 of 10(page number not for citation purposes)tion (mean change of ... involvement with onset of symptoms within 1 to 8 weeks of exposure.The frequency of the syndrome is estimated at 10–100 per100,000 persons exposed. Risk of serious hypersensitivityreactions...
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Báo cáo Y học: Identification and characterization of thioredoxin and thioredoxin reductase fromAeropyrum pernixK1 pdf
... activitywas measured using the glutathione-disulfide transhydro-genase assay described by Gan et al.[25].Thioredoxin reductase activity assaysAssays for thioredoxin reductase activity were ... coding for aprotein of 349 amino acids. Amplification was carried out at94 °C for 30 s, 55 °Cfor2s,74°C for 30 s for 30 cycles using KOD DNA polymerase. The plasmid pET-3d wasthen digested with ... Confirmation of the gene sequence in pET-3dwas carried out by DNA sequencing, using the ABI Prism310 genetic analyzer of Applied Biosystems (Foster city,CA, USA). E. coli BL21(DE3) cells were transformed...
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Báo cáo y học: "Gender difference in QTc prolongation of people with mental disorders" docx
... Annals of General Hospital Psychiatry 2004, 3 http://www.general-hospital-psychiatry.com/content/3/1/3Page 4 of 6(page number not for citation purposes)Table 3 shows the results of one-way ANCOVAs. ... related factors of the QTc prolongation of people with mental disorders.MethodsSubjectsWe retrospectively reviewed the medical charts of patients with history of previous psychiatric treatment, ... 1National Institute of Public Health, 2-3-6 Minami, Wako, Saitama 351-0197, Japan, 2Department of Psychiatry, Division of Neuroscience, Graduate School of Medicine, The University of Tokyo, Japan and...
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Báo cáo y học: "Is Obesity Associated with an Increased Risk for Airway Hyperresponsiveness and Development of Asthma" pdf
... Section of Allergy andImmunology, Department of Internal Medicine; and Mary Cheang:Department of Community Health Sciences, University of Manitoba,Winnipeg, MB.This study was supported by a grant ... Obesity and Asthma 5745. Jarvis D, Chinn S, Potts J, et al. Association of body mass index with respiratory symptoms and atopy: results from the EuropeanCommunity Respiratory Health Survey. Clin ... Additionally,subjects with a history of smoking ($ 10 pack-years),chronic steroid use, and suspected restrictive pulmonarydisease (forced vital capacity [FVC] , 80% predicted andratio of forced...
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