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Báo cáo khoa học: "Silviculture of high-quality oaks: questions and future research needs" docx

Báo cáo khoa học:

Báo cáo khoa học: "Silviculture of high-quality oaks: questions and future research needs" docx

... articleSilviculture of high-quality oaks: questions and future research needsGT WeaverH Spiecker1 Department of Forestry, Wildlife and Fisheries, The University of Tennessee, ... of stands, seed sources and, in theUnited States, to repeated high-grading of stands. Assessment of genetic status of stands and techniques for genetic improve-ment of ... origins and past treatment of these stands are oftenuncertain and increase the uncertainty of preparing appropriate and effective pre-scriptions.The history of present stands...
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Tài liệu Báo cáo khoa học: Plasticity of laccase generated by homeologous recombination in yeast docx

Tài liệu Báo cáo khoa học: Plasticity of laccase generated by homeologous recombination in yeast docx

... mL)contained 10 lL of appropriately diluted enzyme sample and 980 lL of Mes buffer (50 mm, pH 5.7), and 10 lLof0.8 mgÆmL)1SGZ in MeOH was added to initiate the reac-tion. One unit (U) of laccase ... inducedswapping of amino acids for 94 positions (19% of theresidues) in the original LAC3 sequence. The C- and N-termini of the hybrids were 33–37% and 9–26%different, respectively, from that of LAC3.Expression, ... that of LAC3 (Fig. 5). On the other hand,LAC131 and LAC232 hybrids oxidize ABTS 31- and 5-fold faster, respectively, than the parental LAC3enzyme at pH 8.0. As the kinetic behaviour of all of our...
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Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx

Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx

... thebinding of substrates and other inhibitors and to iden-tify the mechanism by which it regulates the activity of cathepsin B.ResultsPreparation and characterization of 2A2 mAb and its Fab ... Jevnikar1, Gregor Anderluh3 and Janko Kos1,21 Faculty of Pharmacy, University of Ljubljana, Slovenia2 Department of Biotechnology, Jozef Stefan Institute, Ljubljana, Slovenia3 Department of Biology, ... prox-imity of the active site, which causes inhibition of cathepsin B endopeptidase activity and activation of its exopeptidase activity.The dual activity of cathepsin B is a consequence of the...
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Tài liệu Báo cáo khoa học: Unfolding of human proinsulin Intermediates and possible role of its C-peptide in folding/unfolding pptx

Tài liệu Báo cáo khoa học: Unfolding of human proinsulin Intermediates and possible role of its C-peptide in folding/unfolding pptx

... nativePAGE.Isolation and purification of the scrambled disulfideisomers of HPIIn the presence of denaturant and thiol catalyst asindicated above, HPI was converted into the mixture of native and scrambled ... concentration of denaturant.Fig. 3. Unfolding of HPI in the presence of denaturant and thiol cata-lyst. Controls for the disulfide scrambling method include the lack of thiol catalyst and lack of denaturant ... sequences and in vitro refolding pathway of PIP and HPI. (A) Amino acid sequences of PIP and HPI. Amino acids areshown in the one-letter code. The numbering of the residues in HPI and PIP are...
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Tài liệu Báo cáo khoa học: Distribution of class I, III and IV alcohol dehydrogenase mRNAs in the adult rat, mouse and human brain ppt

Tài liệu Báo cáo khoa học: Distribution of class I, III and IV alcohol dehydrogenase mRNAs in the adult rat, mouse and human brain ppt

... genes ADH1, ADH3 and ADH4.Similar mRNA length and high nucleotide and aminoacid sequence identity of all ADHs lead to a large risk forcross-reactivity of probes at the mRNA and protein level,making ... cells and Purkinje cells, in the hippocampal formation and differentregions of the cerebral cortex. ADH4 mRNA expressionwas found in Purkinje cells and white matter of thecerebellum, and in ... expressionlevels were found in neurons of the red nucleus in humantissue, while cortical neurons, pyramidal and granule cells of the hippocampus and dopamine neurons of substantianigra showed moderate...
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Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx

Báo cáo khoa học: Enzymes of creatine biosynthesis, arginine and methionine metabolism in normal and malignant cells Soumen Bera1, Theo Wallimann2, Subhankar Ray1 and Manju Ray1 potx

... prominent up-regulation of the expression and enzymatic activity of both AGAT and GAMT. The specific activity of AGAT in sarcomatissue reached almost 50% of that observed in thekidney and it was also ... promoteornithine and SAH production, which in turn could beused for the formation of polyamines and methionine.An investigation of the level of different intermediatemetabolites and the expression of different ... samples and also in theliver, but remained undetectable with this method innormal muscle.RT-PCR and mRNA expression analysis of AGAT and GAMTBoth measurements of catalytic activity and immuno-blot...
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Báo cáo khoa học: Characterization of natural vasostatin-containing peptides in rat heart docx

Báo cáo khoa học: Characterization of natural vasostatin-containing peptides in rat heart docx

... containing bands c, d and g at 45, 37 and 27 kDa, respectively, correspondsto shorter N-terminal CGA-derived fragments. Thethird group is composed of weakly labeled bands (e, f,h, i, j and k labels) ... Isolation and identification of intact chromogranin A and two N-terminal processingproducts, vasostatin I and II, from bovine adrenalmedulla chromaffin granules by chromatographic and mass spectrometric ... Strasbourg, France4 Department of Biological and Technological Research, San Raffaele H Scientific Institute, Milan, ItalyChromogranin A (CGA) is the major member of afamily of acidic glycoproteins,...
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Báo cáo khoa học: Effect of priming on activation and localization of phospholipase D-1 in human neutrophils potx

Báo cáo khoa học: Effect of priming on activation and localization of phospholipase D-1 in human neutrophils potx

... subcellular distribution of PLD and the conse-quences of priming and activation were determined usingimmunoprecipitation and Western blot analysis of neutro-phil cytosol and membrane fractions using ... the activation of the neutrophil respiratory burst. However, the repertoire of PLD isoforms present in these primary cells, the precisemechanism of activation, and the impact of cell priming ... production and for thestudy of the activation of the generating system. J. Clin. Invest. 61,1081–1087.19. Randall, R.W., Bonser, R.W., Thompson, N.T. & Garland, L.G.(1990) A novel and sensitive...
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Báo cáo khoa học: Structure of the O polysaccharides and serological classification of Pseudomonas syringae pv. porri from genomospecies 4 doc

Báo cáo khoa học: Structure of the O polysaccharides and serological classification of Pseudomonas syringae pv. porri from genomospecies 4 doc

... namely C3 of RhaII,C2ofRhaIII and RhaIV, and C2 and C3 of RhaIto d 78.5–79.1 (by6–8 p.p.m.), and the similarity of the C2-C6 chemical shifts of the GlcNAc residue to those of nonsubstituted ... whichconsists of four residues of L-Rha and one residue of D-GlcNAc. A relatively large J1,2coupling constant value of 8 Hz for the H1 signal of the GlcNAc residue and theNMR chemical shifts of H5 and ... & Krohn, K. (1997) Demonstration of theimmunochemical diversity of O-chains of lipopolysaccharide of Pseudomonas syringae and inferring of the serogroup- and sero-type-specific epitopes with...
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Báo cáo khoa học: Effects of thymoquinone on isolated and cellular proteasomes pot

Báo cáo khoa học: Effects of thymoquinone on isolated and cellular proteasomes pot

... of Pharaohs: therapeutic potential of black cumin seeds and beyond. Cancer Ther 6, 495–510.4 Shoieb AM, Elgayyar M, Dudrick PS, Bell JL & TithofPK (2003) In vitro inhibition of growth and ... effect of TQ on cell viabilityafter 24, 48 and 72 h of exposure. Cell via-bility was determined by the MTT assay, and is reported as the percentage of viablecells. Each value is the mean ± standarddeviation ... possibil-ity of their use as potential drugs, and numerous stud-ies have been conducted with the aim of findingnatural, nontoxic and inexpensive compounds [36–38].Fig. 6. 20S and 26S proteasome...
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