... higher concentrations of GdnHCl.Autoproteolysis is independent of the proteinconcentration up to 5 m GdnHCl, as concluded fromdegradation kinetics between 1 and 100 lgÆmL )1 pWT(Fig. 4). Hence, ... Ger-many), isopropanol from Sigma-Aldrich Chemie GmbH(Deisenhofen, Germany), and tris(hydroxymethyl)amino-methane (Tris) from Amersham Biosciences (Uppsala,Sweden). All other reagents were ... hydrochloride (GdnHCl) at different protein concentra-tions. pWT [100 lgÆmL )1 (s), 50 lgmL )1 (e), 20 lgmL )1 ( ,), 5 lgmL )1 (h) and 1 lgmL )1 (n)] was incubated in 50 mMTris ⁄ HCl, pH 7.5,...