... microorganism, Photorhabdus. First, cleavage of three biologicalpeptides, the A and B chains of insulin and b-lipotropin, and of 15 syntheticpeptides, was investigated. In the biological peptides, a preference ... [1].Identification of PrtA cleavage sites in biologicalpeptidesInsulin A and B chains and b-lipotropin were digested withPrtA at 30 °C, in 50 mm Tris ⁄ HCl buffer (pH 8.0) contain-ing 10 mm CaCl2 and 0.1 ... (1995) Themetzincins – topological and sequential relationsbetween the astacins, adamalysins, serralysins, and matrixins (collagenases) define a superfamily of zinc-peptidases. Protein Sci 4, 823–840.20...