... [10,11,15,31]. Previously,the production of H2O2was considered to be the dis-tinctive property of NOX proteins from thermophiles[10,11], with the exception of NOX from P. furiosus,which produces ... proceduresPurification of NOXtp from T. profundusThermococcus profundus cells (8 L) were grown at 80 °Casreported previously [32]. After harvesting, the cells were dis-solved in 20 mm potassium phosphate ... spectral features of proteins with bound flavincofactors (Fig. 4A). The absorbance behavior alsoallowed the determination of the number of flavin mol-ecules bound per mole of NOXtp subunit [17,25,26]....