Báo cáo khoa học: "Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand" potx

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... Y. Cai et al.3584FEBS Journal 276 (2009) 35753588 ê 2009 The Authors Journal compilation ê 2009 FEBS Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 Yuanheng ... subunit molecular masswas also estimated by SDS–PAGE. Characterization and comparative analyses of HYDJs and HYDBpThe optimal temperature for activity of HYDJswith d-p-HPH as substrate was ... residues are shaded black, and the residues relevant to metal ion binding are indicated by ﬁlled stars.Y. Cai et al. A novel high-activity D-hydantoinase from Jannaschia sp. CCS1 FEBS Journal 276...

Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx

... 5Â-CTTTATTTTCAGGGCGCCATGAAGCGCGCAAGACCGTCTGAA-3Â and a reverse oligomer 5Â-AGCTCGAATTCG GATCCGGTACCTCAGAAGGTAGACAGCAGAACC-3Â. For derivitization with TMR, a Gly-Gly-Cyssequence was introduced at the C-terminus ... addition to an N-terminal Nco1 cloning site.The forward o ligomer 5 Â-TCCGAAACCAGCG GCCGCTTTATCGCGTTA AAAC CGGT GATCAA ACCCC -3Â and thereverse oligomer 5Â-GTAGGCCTT TGAATTCCTCAAAAAGTGCGGCTCGAT-3Â ... oligomers5Â-CTGCTGTCTACCTTTGGAGGTTGCTGATCCGAATTCGAG-3Â (forward) and 5Â-GCTCGAATTCGGATCAGCAACCTCCAAAGGTAGACAGCA-3Â (reverse). BL21cells were transformed with the pETM11 construct and grown until a D of 0.8...

Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc

... from the archaea kingdom, indicatingthat the enzymatic activities of the MAPEG family arenot present in these species or that these activities arecatalysed by other enzymes. The absence of MAPEG members ... [57].Isolation and cloning of the SynMGST and E.coliMGST The coding sequence for the SynMGST, corresponding to the complementary strand of the nucleotide sequence from89 254 to 89 667 in the Synechocystis ... above and in[1]). We investigated representative gene products from the E. coli and Synechocystis sp. bacteria further, togain insight into the function of these proteins and the evolution of the...

Tài liệu Báo cáo khoa học: Biochemical and molecular characterization of hazelnut (Corylus avellana) seed lipoxygenases pdf

... organoleptic features of hazelnut seeds, we carried out a study to characterize hazelnut lipoxygenases at the biochemical and molecular level.Materials and methodsPlant material Hazelnut (Corylus avellana ... physiologicalrole of seed LOXs is still unclear. With the aim to betterclarify the occurrence of LOXs and their inﬂuence on hazelnut seed quality, we carried out the biochemical and molecular characterization ... primers CA4 and CA5 (matching,respectively, from base 9488 to base 9520 and from base8366 to base 8387 of the sequence reported in Fig. 1) and Ó FEBS 2003 Characterization of hazelnut seed lipoxygenases...

Báo cáo khoa học: Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus doc

... conserved and unique components of the Cag- T4SS, together withpotential implications for the current understanding of its mode of action. The cagPAI and the Cag type IV secretion apparatus Gene ... 2011 The Author Journal compilation ê 2011 FEBS MINIREVIEW Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus Wolfgang FischerMax von Pettenkofer-Institut, ... components, see the accom-panying review by Cendron and Zanotti [27]. The putative type IV secretion apparatus corecomplex The assembly of different type IV secretion machines and functions of their...

Báo cáo khoa học: Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution pptx

... compilation ê 2009 FEBS 1401 Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl–tRNA synthetase and determination of its oligomeric structure in solution Stefano Paravisi1, ... minor, implying rotations of domains of just a few degrees (e.g. 7° interdomain rotations upontRNA binding to the Tt-GluRS and local limitedrearrangements in the active site) [5]. Interestingly, ... thediscriminating behaviour of Mt-GluRS in E. coli.Studies on Tt-GluRS indicated that discriminating and nondiscriminating GluRS can be distinguished onthe basis of the presence of a speciﬁc...

Báo cáo khóa học: Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea docx

... various fungal laccases.326 S. Chen et al. (Eur. J. Biochem. 271) Ó FEBS 2003 Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea Shicheng ... oneother laccase isoform in addition to lac1 [1], this and possibly other laccase isoforms may be contributing to the total laccase activity detected at the various developmentalstages. The pattern ... addedFA.Transcriptional regulation of lac1during growth and fruiting of V. volvacea on straw The duration of the developmental cycle of V. volvacea when the mushroom was grown on rice-straw...

Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the ﬁrst ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt

... protocol of RIP purification. As Fig. 1 shown, the CM-cellulose 52 column retained the most of the RIPactivity and also separated the lamjapin from the otherimpurities. The proteins obtained from the ... pooled and subjected to furtherpurification.4748 R s. Liu et al. (Eur. J. Biochem. 269) Ó FEBS 2002 Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from ... as the distribution of this class of protein in plant kingdom.MATERIALS AND METHODSMaterials The fresh tender leaves of kelp (Laminaria japonica A) werecollected in winter at the shore of...

Báo cáo khoa học: Functional and genetic characterization of the promoter region of apolipoprotein H (b2-glycoprotein I) ppt

... the functional roles of the )643T>C and )1219G>A SNPs as part of the extended APOH promoter transcriptional machinery.To further substantiate the functional relevance of the three APOH promoter ... quantitativechange at the protein level. Whether the APOH pro-moter SNPs ( )643T>C and )1219G>A) could inﬂu-ence the promoter activity by either the former orlatter methods is beyond the scope of ... cross-speciesconservation of the APOH promoter sequence.ResultsIdentiﬁcation and characterization of the APOH promoter region In order to localize the active promoter region and toidentify regions that are...

Báo cáo khoa học: Functional and structural characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deﬁcient families from Southern Italy docx

... characterization of novel mutations and genotype–phenotype correlation in 51 phenylalanine hydroxylase deﬁcient families from Southern Italy Aurora Daniele1,2,3, Iris Scala4, Giuseppe Cardillo1,5, Cinzia ... analysis of thePAH gene in 51 unrelated HPA patients from South-ern Italy. In addition to the molecular epidemiology of PAH mutations, we characterized the functional prop-erties of two novel mutations ... severe form of hyperphenylal-aninemia is classic phenylketonuria. We sequenced the entire gene for phenylalanine hydroxylase in 51 unrelated hyperphenylalaninemia patients from Southern Italy. The...

Báo cáo khoa học: Structural and functional characterization of human Iba proteins ppt

... cross-linking efficiency of Iba1 and Iba2 or in the overall morphology of the generatedfilament bundles.Calcium affinity of Iba1 and Iba2 Homodimerization and actin binding of Iba1 and Iba2 were similar ... presented here reveals functional simi-larities and differences between Iba1 and Iba2 . Weinvestigated Ca2+binding and homodimerization of Iba1 and Iba2 . Furthermore, F-actin binding and cross-linking ... may be concluded that EF-hand 1 of the Iba proteins is not capable of functional Ca2+binding.Function of EF-hand 2In both Iba1 crystal structures, the loops of EF-hand 2adopt essentially the...

Báo cáo khoa học: Biochemical and molecular characterization of puriﬁed chicken pancreatic phospholipase A2 docx

... FEBS Biochemical and molecular characterization of puriﬁed chicken pancreatic phospholipase A2Aida Karray, Fakher Frikha, Abir Ben Bacha, Yassine Ben Ali, Youssef Gargouri and Soﬁane BezzineLaboratoire ... zymogen of phospholipase A in human pancreatic juice. BiochimBiophys Acta 227, 213–217.12 Evenberg A, Meyer H, Verheij HM & de Haas GH(1977) Isolation and properties of prophospholipase A2 and ... model of group IB ChPLA2was built using the porcine pancreatic phospholipase A2structure as template.AbbreviationsChPLA2, chicken pancreatic phospholipase A2; DrPLA2, dromedary pancreatic...

Báo cáo khoa học: "Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods" pdf

... uberis strain Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis 223studies on Streptococcus uberis types I and II. Description of Streptococcus ... cloning of a streptokinase from Streptococcus uberis . Infect. Immun. 1999, 67, 1072-1078. Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis ... (2003),/4(3), 213–223Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methodsI. U. Khan1, A. A....

Báo cáo khoa học: "Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand" potx

... strain of infectious bronchitis virus isolated in the UK. Vet Rec 2008, 162, 99-100. 11. Gough RE, Randall CJ, Dagless M, Alexander DJ, Cox WJ, Pearson D. A ‘new’ strain of infectious bronchitis ... region of the S1 gene of infectious bronchitis virus. Arch Vrol 2000, 145, 291-300.21. Yu L, Wang Z, Jiang Y, Low S, Kwang J. Molecular epidemiology of infectious bronchitis virus isolates from ... and molecular characterization of infectious bronchitis virus 221Fig. 1. Phylogenetic tree based on the nucleotide sequence between aligned S1 sequences from Thai infectious bronchitis virus...

Báo cáo khoa học: " Complete genomic sequence analysis of infectious bronchitis virus Ark DPI strain and its evolution by recombination" pot

... AbstractAn infectious bronchitis virus Arkansas DPI (Ark DPI) virulent strain was sequenced, analyzed and compared with many different IBV strains and coronaviruses. The genome of Ark DPI consists of 27,620 ... 1 of 7(page number not for citation purposes)Virology JournalOpen AccessShort report Complete genomic sequence analysis of infectious bronchitis virus Ark DPI strain and its evolution by ... proteins and because of unavailability of sequence information for rest of the genome; we speculate thatGA98 may be a derivative of the Ark DPI strain. Analysis of the structural region of Ark DPI...

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