... recy-cling. Initiation is the rate-limiting step [1] . This phaseis mediated by initiation factor 1 (IF1), initiation fac-tor 2, and initiation factor 3. IF1 is the smallest of the initiation factors ... chloramphenicol; IF1, initiation factor 1; Kan, kanamycin; Tet, tetracycline; TIR, translation initiation region. FEBS Journal 278 (2 011 ) 17 4 517 56 ê 2 011 The Authors Journal compilation ê 2 011 FEBS ... over-expression of era, encoding the GTPase Era. The primersused were as follows: era_F_NcoI, CGACCATGGCGAACAGGCGTTGAAAAAAC; and era_R_SalI, CGAGTCGACAGCCTTCCATCGGAGTTACT. The resulting vectorwas termed...