... domain is com-posed of two separate helical subdomains (named A andB) connected by random coils. Terminal regions of sequential domains contribute to the formation of inter-domain helices linking ... helices linking domain IB to domain IIA, anddomain IIB to domain IIIA, respectively [3,7,11,13–21].The structural organization of HSA provides a variety of ligand-binding sites. The heme binds physiologicallyKeywordsferric ... !ð4bÞ½FeðIIÞÀNOt¼½FeðIIIÞiÀ½FeðIIIÞtþ½FeðIIIÞÀNOtð4cÞValues of konand koff(reaction (a) in Scheme 1) weredetermined from the dependence of kobson [NO], accordingto Eqn (2) [44].Values of K (= koff⁄ kon; reaction (a) in Scheme 1)...