Báo cáo khoa học: "Autologous somatic cell nuclear transfer in pigs using recipient oocytes and donor cells from the same animal" pdf

... obtained from autolo-gous SCNT using oocytes and cumulus cells from the same pig than from SCNT using autologous follicular cells and heterologous skin fibroblasts (5 ± 2% and 10 ± 1%). There ... 415󰠏421*Corresponding authorTel: +82-33-250-8670; Fax: +82-33-244-2367E-mail: eslee@kangwon.ac.krAutologous somatic cell nuclear transfer in pigs using recipient oocytes and donor cells from the same ... objective of the present study was to examine the feasibility of the production of autologous porcine somatic cell nuclear transfer (SCNT) blastocysts using oocytes and donor cells from slaughtered...

Báo cáo khoa học: "Expression of cell cycle proteins in male breast carcinoma" docx

... is the corresponding and first author who was involved in the design and implementation of the study with overseeing of the data gathering and data interpretation. IF and TR are international exchange ... (76%-100%)% positive stained cells in tumourFigure 1 Percentage of Cell cycle protein expression in the tumor cells. X-axis displays: the expression of the cell cycle proteins CyclinD1,p21, Ki67, ... niversalcyclin-dependent kinase inhibitory protein (CDK1) withan affinity for G1 and G2 cyclin-CDK complexes, thusacting as “checkpoint proteins” at the G1 and G2 levels(figure 6). The molecular machinery...

báo cáo khoa học: "Improved retroviral suicide gene transfer in colon cancer cell lines after cell synchronization with methotrexate" docx

... in synchronizing DHDK12 cells in S phase thanara-C or aphidicolin. Consequently, the efficacy of MTX in synchronizing cells in S phase was then tested i n the HT29 cell line. In HT29 cell line, ... 12following MTX removal. This combination of anincrease of cells in S phase and a decrease of cells in G1phase resulted in a wave of cells in G2-M between 10 and 24 hr after MTX removal. The synchronization ... ction increased in cells synchr onized in S phase. The highest level of transduction was obtained in the cells infected 20 hr after MTX removal. At that time, the proportion of transduced cells...

Tài liệu Báo cáo khoa học: "Toward finer-grained sentiment identification in product reviews through linguistic and ontological analyses" ppt

... too. In the future, we need to assess the importance of each proposed clue relative to others and to the existing ones. We also need to investigate the nature of interactions among linguistic, ... these clues can discriminate between BP and P and discriminate between AN and N. The regression equation for distinguishing AN from N is statistically significant at the 5% level (F(7,177) ... some of them as attributes with the following guidelines and filtered out the rest: 1) NP with frequent adjectives 2) NP with frequent non-functional and intransitive verbs. In the case of...

Tài liệu Báo cáo khoa học: "Finding Hedges by Chasing Weasels: Hedge Detection Using Wikipedia Tags and Shallow Linguistic Features" doc

... Others argue {{weasel-inline}} that the news media are simply cateringto public demand.(2) therefore America is viewed bysome {{weasel-inline}} technologyplanners as falling further behindEurope ... Preceding Weasel TagsWe investigate the ﬁve words occurring right be-fore each weasel tag in the corpus (but within the same sentence), assuming that weasel phrases con-tain at most ﬁve words and ... Opinion mining and sen-timent analysis. Foundations and Trends in InformationRetrieval, 2(1-2):1–135.Riloff, Ellen, Janyce Wiebe & Theresa Wilson (2003). Learn-ing subjective nouns using...

Báo cáo khoa học: Dissociation/association properties of a dodecameric cyclomaltodextrinase Effects of pH and salt concentration on the oligomeric state pot

... dimer in both the solution and crystal states [3]. The C-domain was, however,shown to be distinctly separated from the active sitegroove and was not involved in main-chain to main-chain hydrogen ... bonding with either the N- or the (b/a)8-barrel domain. Interestingly, the C-terminaldomain was found to be critically involved in the supramolecular assembly of CDase [6]. In this study, we investigated ... the information obtained about the 3D struc-ture of CDase I-5, the quaternary state of CDase I-5was likely to be maintained by the intrinsic capabilityof the N- and C-terminal regions of the...

Báo cáo khoa học: Theoretical study of lipid biosynthesis in wild-type Escherichia coli and in a protoplast-type L-form using elementary ﬂux mode analysis potx

... caused by the loss of the cell wall and the outer membrane.Impact of the deﬁciencies in the cell wall-freemutant In the cell wall-free mutant, two genes of lipid biosyn-thesis contain synonymous ... the studied enzymescontain mutations in the cell wall-free mutant. Byexamining the mutations in the corresponding genes,we were able to deduce which proteins might still befunctional in the ... preserved the 51 EFMs for the production oflipid A (ca) present in the intact system. The require-ments for ATP and NAD(P)H of these modes are the same as in the unperturbed system. Data from the...

Báo cáo khoa học: Three-dimensional structure of a thermostable native cellobiohydrolase, CBH IB, and molecular characterization of the cel7 gene from the ﬁlamentous fungus, Talaromyces emersonii ppt

... performing 5¢ -and3 ¢-RACE PCRs by using the outer and inner RACE primers supplied by the manufacturer and the o uter and inner gene-speciﬁc primersdesigned from the cel7 PCR products. The Cel7 ... residue in site )5, thus forming the lid of the binding site. The structures 1Q9H and 1GPI have a deletionof these three residues, thus leading to a more open substrate-binding site.Catalytic binding ... nativeglycoprotein and cellulase from this thermophilic eukaryote. The long cellulose-binding tunnel seen in GH7 Cel7A from Trichoderma reesei is conserved in 1Q9H, as are the catalyticresidues....

Báo cáo khoa học: "Robust Dialog Management with N-best Hypotheses Using Dialog Examples and Agenda" pdf

... dialog using the agenda graph and 10-best hypotheses. From these results, we con-clude that the the use of the n-best hypotheses with the agenda graph is helpful to improve the robust-ness of the ... to maximize the examplescore in the best node. The generation of candidate nodes is basedon multiple hypotheses from the previous EBDM634between the hypothesis score Sh and the discoursescore ... nodes.S,H ,and G denote the focus stack, hypothesis, and agenda graph, respectively. The INTERPRET al-gorithm is initially called to interpret the current dis-course state. Furthermore, the essence...

Báo cáo khoa học: "Efﬁcient Unsupervised Discovery of Word Categories Using Symmetric Patterns and High Frequency Words" ppt

... 100.Corpus window size was determined by starting from a very small window size, deﬁning at ran-dom a single window of that size, running the al-gorithm, and iterating this process with increasedwindow ... stopwords from the corpus, and then used as features the words whichappear before or after the target word. In the calcu-lation of feature values and inter-vector distances, and in the removal ... of theseat random. 10 triplets were selected in the same manner from the categories produced by k-means, and 10 triplets were generated by random selec-tion of content words from the same window...

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