... S. cerevisiae vector control. Group C: Oral vaccination with S. cerevisiae expressing ApxIA antigen. Grou
p
D: Oral vaccination with S. cerevisiae expressing ApxIIA antigen. Group E: Combined oral ... immune responses by oral vaccination with
Saccharomyces cerevisiae expressing recombinant Actinobacillus
pleuropneumoniae ApxIA or ApxIIA...
... the induction of
pro-inflammatory cytokines and granulocytes into the
lungs following either intramuscular or intranasal VLP
vaccination.
The recombinant influenza virus-like particle vaccines
preserve ... elicited by vaccinationFigure 1
Antibodies elicited by vaccination. Mice (n = 8) were
vaccinated via intramuscular injection at weeks 0 and 3 with
VLPs (clade 1 or clade...
... N-terminal domains (N1, N2) of the minor coat
protein (g3p) are responsible for binding and infection in E. coli.Thus,
incorporation of a library of target proteins between the N-terminal
domain ... Protein design by binary patterning of polar and non-
polar amino acids. Science 262, 1680–1685.
8. Michnick, S.W. (2001) Exploring protein interactions by inter-
action-induced fold...
... use of leucine,
isoleucine, valine, methionine, tyrosine, tryptophan or
phenylalanine as a sole nitrogen source, leads to the
formation of the fusel alcohols and acids (Fig. 1) [1].
Indeed, in Saccharomyces ... of N-terminal methionine from recom-
binant proteins by engineered E. coli methionine amino-
peptidase. Protein Sci
13, 1802–1810.
29 Duggleby RG (2006) Domain relationsh...
... Japan
2
Laboratory of Biopharmaceutical Research, National Institute of
Biomedical Innovation, 7-6-8 Saito-asagi, Ibaraki, Osaka 567-0085, Japan
3
The
Center for Advanced Medical Engineering and Informatics, ... such as nSP30
would induce Th2-polarized allergic immunity. Our
results support previous reports showing that the
immune- activating effect of nanomaterials increases
with de...
... an important role in protection of neuronal cells
against stress-induced apoptosis [26]. In accordance with
these findings, ischemia/reperfusion in the rat forebrain
induces the expression of HSP105/110 ... activation of p38 induced by
oxidative stress, we further examined whether its activation
is responsible for the induction of apoptosis in F9 cells using
SB202190, a pot...
... studied by means of capillary e lectrophoresis
combined with laser-induced fluorescence detection, confo-
cal laser scanning microscopy and fluorescence-activated cell
sorting. Incubation with the fluorescein-labelled ... ,
respectively.
Results
Conjugation with MAP leads to an increased intracellular
availability of PNA
In order t o e xamine the ability of M AP to de live r PNA...
... alkylating agents by linking the alkylating
pharmacophore to the DNA-affinity molecules (e.g.,
DNA intercalating agents, DNA minor groove binder)
[7,8]. In most cases, the DNA-directed alkylating ... irradiated with 2 Gy of g-rays. The
Annexin V/PI staining was then determined with FACS
analysis. Cells treated with either 200 nM of BO-1051
alone or combined with irradiation...
... a-synuclein. For this,
a-synuclein was incubated in the presence of MPTP and MPP
+
along with
dopamine. The fibrillation of a-synuclein was monitored by Thioflavin T
fluorescence and immunoblotting. ... occur in the brain include
selective loss of dopaminergic neurons in substantia
nigra pars compacta and appearance of Lewy bodies
consisting of aggregated protein, mainly a-sy...
... inhibitors, binding revers-
ibly into the active site of the enzyme. Cystatins,
including human cystatin C, are general inhibitors of
cysteine proteases. For cathepsin B, their K
i
value is in
nanomolar ... mech-
anism applies to the binding of cystatin C to cathepsin
B, which takes place in two steps: an initial weak inter-
action with N-terminal region of the inhibitor inducin...