... Ozawa Y, Nakamura T, Kamata N, Yasujima D,Urushiyama A, Yamakura F, Ohmori D & Imai T(2005) Thermococcus profundus 2-ketoisovalerateferredoxin oxidoreductase, a key enzyme in the archaealenergy-producing ... coupledassay. In the presence of the reduced Fd1 and acetyl-CoA, the HE-TPP radical intermediate was formed(Figs 3F and 4B), indicating the intermediacy of theHE-TPP radical in both the oxidative and ... were involved in the two reactions catalyzed by POR and OGOR(Fig. 1, dashed arrows). In particular, CoA was a sub-strate of OGOR as well as a product of POR, and sig-nificantly affected the reaction...