... summarisa-
tion and MT to be carried out in a month on
a surprise language, the nature of which will
not be known in advance. The open and flex-
ible architecture of GATE, and the separation
of linguistic ... Bulgarian named entities in GATE
of the gazetteer lists (e.g. lists of first names,
days of the week, locations etc. were tailored for
Bulgarian), and in terms of s...
... (Arg21, Arg111, and His138) and with the
carboxylate end (Arg21 and Arg73) of GriP. Of partic-
ular note is a short, 2.46-A
˚
hydrogen bond between N-
e of His138 and a phosphate oxygen of GriP.
Alanine ... some of
the key findings that have led to our current under-
standing of the molecular mode of action of glypho-
sate and the molecular basis for glyphosate resist...
... open
questions. The use of mutant (e.g. deletion and extra
copy numbers) and transgenic (RNAi and overexpres-
sion) lines will help us to better understand the
relationship between Hsps and cold tolerance.
Experimental ... with
$ 10 mL of standard food medium. Flies of the RA group
were transferred to vials containing $ 10 mL of 1% agar,
which provides a source of water but...
... site. A NOESY spectrum of 2
and 3 in the presence of a small quantity of protease
revealed an intermolecular cross-peak between the
methyl group of 3 and the phthalazine ring of 2,as
expected for ... spectra of the com-
pounds 2 and 3 in the absence and presence of WNV
NS2B–NS3pro(K96A) in D
2
O solution containing
1.5% d
6
-dimethylsulfoxide.
Fig. S5. Superimposition o...
... a
mixture of annexin A1 and A2 and that the 35 kDa proteins are a mixture of annexin A5 and proteolytic fragments of annexin A1 and annex-
in A2. Purified recombinant annexin A1, annexin A2 and annexin ... show the sites cleaved and the mole-
cular masses of the rest of the cleaved peptides. Arrows show the
N-termini of the 35 kDa forms of annexin A1 and annexin A2....
... loss of enzyme activity through imprinting, immo-
bilizing and crosslinking of the native enzyme. The
temperature stability of the immobilized and CLIP
CGTases from A11 and BM at 60 °C and 70 ... Pathumthanee, Thailand
2 Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, University of Leipzig, Germany
3 Starch and Cyclodextrin Research U...
... position of the absorbance peak (280 nm) of the stand-
ard marker with that of aspartyl aminopeptidase.
Effect of inhibitors on aspartyl aminopeptidase
activity
Solutions of various inhibitors of enzymatic ... generated was estimated from the
ratio of the height of the product peptide peak versus
the sum of the heights of the product peptide and sub-
strate peaks.
Purific...
... by a factor of
approximately two [25]. T hirdly, a ctivity of D 30A was
weakly modified only after irradiation w ith 100 Gy, while
that of WT and of W35A increased by a factor of 1.5 and
1.2, respectively ... Trx, D30A and W35A
after oxidation with a dose of 100 Gy. For each form, the
chromatograms were very similar and showed the forma-
tion of a s ingle major product. T...
... assays,
fluorescence titrations and analytical gel filtration.
Results and Discussion
Hydrolytic activity of hGBP5a/b and hGBP5ta
In light of previous observations for hGBP1 and other
large GTPases, ... with Hill coefficients of 2.4 and 1.9 and dissoci-
ation constants (K
d
Hill
) of 4 and 2 lm for hGBP5a ⁄ b
and hGBP5ta, respectively. The proteins are very similar
in...