Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx

Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx

Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx

... (2005), / 6 (1), 7–19 Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD Ji-Yeon Kim 1,2 , So-Hyun ... were applied for further determination by multiplex PCR and PCR for flagellar H7 antigen detection. DNA preparation for Multiplex PCR, flagellar H...
Ngày tải lên : 07/08/2014, 18:21
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Tài liệu Báo cáo khoa học: Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing pdf

Tài liệu Báo cáo khoa học: Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing pdf

... were analyzed by autoradiography and silver staining. Equal mobilities of the radioactive and the protein-containing bands indicated covalent attachment of DNA to the enzyme. The yield of the photochemical ... action of base release by Escherichia coli Fpg protein: role of lysine 155 in cata- lysis. Biochemistry 36, 5084–5096. 21. Smith, R.A.G. & Knowles, J.R. (1975)...
Ngày tải lên : 20/02/2014, 11:20
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Báo cáo khoa học: Chaperone-assisted refolding of Escherichia coli maltodextrin glucosidase doc

Báo cáo khoa học: Chaperone-assisted refolding of Escherichia coli maltodextrin glucosidase doc

... Government of India, and MHRD, Government of India, for financial support. We also thank Professor Winfried Boos, University of Konstanz, Germany, for the gift of plasmids encoding His-tagged MalZ and ... refolding of Escherichia coli maltodextrin glucosidase Subhankar Paul 1,2 , Shashikala Punam 1 and Tapan K. Chaudhuri 1 1 Department of Biochemical Engineering and Bio...
Ngày tải lên : 16/03/2014, 06:20
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Báo cáo khoa học: Light-induced reactions of Escherichia coli DNA photolyase monitored by Fourier transform infrared spectroscopy pot

Báo cáo khoa học: Light-induced reactions of Escherichia coli DNA photolyase monitored by Fourier transform infrared spectroscopy pot

... of rates and yields of interchromophore (folate fi flavin) energy-transfer and intermolecular (flavin fi DNA) electron-transfer in Escherichia coli photolyase by time-resolved fluorescence and absorption-spectroscopy. ... bands at 1532 and 1396 cm )1 , and changes in the amide-I (1600–1700 cm )1 ) region. The frequencies of infrared bands can be modulated by isotope substit...
Ngày tải lên : 16/03/2014, 18:20
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Báo cáo khoa học: Aglycone specificity of Escherichia coli a-xylosidase investigated by transxylosylation ppt

Báo cáo khoa học: Aglycone specificity of Escherichia coli a-xylosidase investigated by transxylosylation ppt

... showed a correlation between C-1¢ of the xylosyl moiety and H-6 of the fructosyl moiety, and the other showed a corre- lation between C-1¢ of the xylosyl moiety and H-3 of the fructosyl moiety in the ... catalytic domain of each monomer is a triose-phosphate isomerase barrel flanked by two b-sandwich structures. The glycone-binding site is formed by the C-terminal ends of...
Ngày tải lên : 23/03/2014, 07:20
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Báo cáo khoa học: Cd2+-induced aggregation of Escherichia coli pyrophosphatase doc

Báo cáo khoa học: Cd2+-induced aggregation of Escherichia coli pyrophosphatase doc

... Physico-Chemical Biology and School of Chemistry, Moscow State University, Moscow, Russia; 2 Department of Biochemistry, University of Turku, Finland We report here that Escherichia coli pyrophosphatase aggregates ... ligands – Asn24 and Asp26 – from two subunits Fig. 1. Time-course of Escherichia coli inorganic pyrophosphatase (PPase) aggregation in the presence of 2 m...
Ngày tải lên : 23/03/2014, 13:20
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Báo cáo khoa học: Mammalian transglutaminases Identification of substrates as a key to physiological function and physiopathological relevance pot

Báo cáo khoa học: Mammalian transglutaminases Identification of substrates as a key to physiological function and physiopathological relevance pot

... 399 of one chain and Lys406 of another fibrin. Successively, the enzyme sta- bilizes the blood clot by cross-linking lysine and gluta- mine of the a and c chains of fibrin molecules [24]. Sobel and ... cross-linking of proteins mediated by TG isoforms results in well-defined functions in various biological processes: cross-linking of fibrin in haemostasis, of semenogelin...
Ngày tải lên : 30/03/2014, 15:20
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Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc

Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc

... 100 lm of (GlcNAc) 1–4 was analysed at the start, in the middle and at the end of each series of sam- ples. The resulting average values of the standards (display- ing standard deviations of < ... Materials and methods) . (D) Chromatogram of (GlcNAc) 6 degradation products generated by LlChi18 after 2 min of incubation with 1 n M of enzyme. The double peaks represent...
Ngày tải lên : 18/02/2014, 08:20
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Tài liệu Báo cáo khoa học: Three-dimensional structures of thermophilic b-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa Comparison of twelve xylanases in relation to their thermal stability pdf

Tài liệu Báo cáo khoa học: Three-dimensional structures of thermophilic b-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa Comparison of twelve xylanases in relation to their thermal stability pdf

... varies between 2 and 12 (Table 6). There is one completely conserved salt bridge between the C-terminal Glu (or Asp) of b-strand B6 (BAX and BCX have Asp) and Arg of the loop between b-strands B7 and A6. ... al.[8],theshapeofthemoleculeresemblesa right hand: two b-sheets and the a-helix form fingers and a palm, a long loop between the B7 and B8 strands forms a thumb, and a...
Ngày tải lên : 20/02/2014, 23:20
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Tài liệu Báo cáo khoa học: A zymogen form of masquerade-like serine proteinase homologue is cleaved during pro-phenoloxidase activation by Ca2+ in coleopteran and Tenebrio molitor larvae docx

Tài liệu Báo cáo khoa học: A zymogen form of masquerade-like serine proteinase homologue is cleaved during pro-phenoloxidase activation by Ca2+ in coleopteran and Tenebrio molitor larvae docx

... proteolysis of the 55-kDa Tm-mas and PO activity by using two kinds of serine proteinase inhibitors, such as p-NPGB and p-APMSF. As shown in Fig. 7C and D, when HW-55S Fig. 6. Localization of the ... concentra- tions were determined by the method of Lowry et al.[16] using BSA as a standard. Molecular cloning of 45-kDa Tm-mas A cDNA library from T. molitor larvae was...
Ngày tải lên : 21/02/2014, 03:20
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