Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
... (2005),
/
6
(1), 7–19
Isolation and identification of
Escherichia coli
O157:H7 using different
detection methods and molecular determination by multiplex PCR and
RAPD
Ji-Yeon Kim
1,2
, So-Hyun ... were
applied for further determination by multiplex PCR and
PCR for flagellar H7 antigen detection.
DNA preparation for Multiplex PCR, flagellar H...
... were
analyzed by autoradiography and silver staining. Equal
mobilities of the radioactive and the protein-containing
bands indicated covalent attachment of DNA to the
enzyme. The yield of the photochemical ... action of base
release by Escherichia coli Fpg protein: role of lysine 155 in cata-
lysis. Biochemistry 36, 5084–5096.
21. Smith, R.A.G. & Knowles, J.R. (1975)...
... Government of India, and
MHRD, Government of India, for financial support.
We also thank Professor Winfried Boos, University of
Konstanz, Germany, for the gift of plasmids encoding
His-tagged MalZ and ... refolding of Escherichia coli
maltodextrin glucosidase
Subhankar Paul
1,2
, Shashikala Punam
1
and Tapan K. Chaudhuri
1
1 Department of Biochemical Engineering and Bio...
... of rates and yields of
interchromophore (folate fi flavin) energy-transfer and
intermolecular (flavin fi DNA) electron-transfer in
Escherichia coli photolyase by time-resolved fluorescence
and absorption-spectroscopy. ... bands at 1532 and 1396 cm
)1
, and
changes in the amide-I (1600–1700 cm
)1
) region.
The frequencies of infrared bands can be modulated
by isotope substit...
... showed a
correlation between C-1¢ of the xylosyl moiety and H-6
of the fructosyl moiety, and the other showed a corre-
lation between C-1¢ of the xylosyl moiety and H-3 of
the fructosyl moiety in the ... catalytic domain of each monomer
is a triose-phosphate isomerase barrel flanked by two
b-sandwich structures. The glycone-binding site is
formed by the C-terminal ends of...
... Physico-Chemical Biology and School of Chemistry, Moscow State University, Moscow, Russia;
2
Department of Biochemistry, University of Turku, Finland
We report here that Escherichia coli pyrophosphatase
aggregates ... ligands – Asn24 and Asp26 – from two subunits
Fig. 1. Time-course of Escherichia coli inorganic pyrophosphatase
(PPase) aggregation in the presence of 2 m...
... 399 of one chain and
Lys406 of another fibrin. Successively, the enzyme sta-
bilizes the blood clot by cross-linking lysine and gluta-
mine of the a and c chains of fibrin molecules [24].
Sobel and ... cross-linking of proteins mediated
by TG isoforms results in well-defined functions in
various biological processes: cross-linking of fibrin in
haemostasis, of semenogelin...
... 100 lm of (GlcNAc)
1–4
was analysed at the
start, in the middle and at the end of each series of sam-
ples. The resulting average values of the standards (display-
ing standard deviations of < ... Materials and methods) . (D) Chromatogram of (GlcNAc)
6
degradation products generated by
LlChi18 after 2 min of incubation with 1 n
M of enzyme. The double peaks represent...
... varies
between 2 and 12 (Table 6). There is one completely
conserved salt bridge between the C-terminal Glu (or Asp)
of b-strand B6 (BAX and BCX have Asp) and Arg of the
loop between b-strands B7 and A6. ... al.[8],theshapeofthemoleculeresemblesa
right hand: two b-sheets and the a-helix form fingers and
a palm, a long loop between the B7 and B8 strands forms a
thumb, and a...
... proteolysis of the
55-kDa Tm-mas and PO activity by using two kinds of
serine proteinase inhibitors, such as p-NPGB and
p-APMSF. As shown in Fig. 7C and D, when HW-55S
Fig. 6. Localization of the ... concentra-
tions were determined by the method of Lowry et al.[16]
using BSA as a standard.
Molecular cloning of 45-kDa Tm-mas
A cDNA library from T. molitor larvae was...