... isopropylamine, n-butylamine, n-amylamine, n-hexylamine, 1,6-diamino-hexane, and spermidine. Interestingly, the enzyme wasunable to use ammonia as a substrate and was distinctfrom alanine dehydrogenase ... Toyobo (Osaka, Japan), and New EnglandBiolabs (Beverly, MA, USA). All other reagents were of analytical grade from Nacalai Tesque (Kyoto, Japan) andWako Pure Chemical Industries (Osaka, Japan).Culture ... N-methyl-l-alanine as thevariable substrate, at a fixed subsaturating concentra-tion of NADP+(0.2 mm), NADPH was a linear mixed-type inhibitor. Pyruvate behaved as a noncompetitiveinhibitor...