... ATCC12633: preincubation at 94 °C for 1 min and then 30 cycles between 98 °C for 20 s and68 °C for 3 min and finally at 72 °C for 10 min. The PCRproduct was digested with NdeI and HindIII and ligatedinto ... NE) from the Ministry of Education, Culture,Sports, Science, and Technology of Japan, by Grant-in-Aid for Encouragement of Young Scientists15780070 (to HM) from the Japan Society for the Pro-motion ... sequentialmechanism for the NMAADH reactions. In fact, the data obtained gave a good global fit to the equation of an ordered Ter Bi mechanism.To test the kinetic mechanism further, we performedinhibition...