**Báo cáo khoa học: "Tissue distribution of bovine viral diarrhea virus antigens in persistently infected cattle Taekyun Shin* and Helen Acland1" ppt**

Tài liệu Báo cáo khoa học: Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells pdf

... enrichment in iNOS activitywithin the particulate fraction of epithelial cells.Apical distribution of iNOS in intestinal epithelialcellsTo get more insight into the localization of iNOS in intestinal ... [18].ResultsSubcellular distribution of iNOS protein and activity in vitroThe distribution of iNOS protein in the cytosol and particulate fraction was examined in DLD-1 cellsexposed to cytokines. To determine ... association of iNOS in vivo In order to determine the distribution of iNOS in intestinal epithelial cells in vivo, experiments were con-ducted in mice injected with bacterial ﬂagellin. Flagel-lin activates...

Báo cáo khoa học: Equilibrium distribution of skeletal actin–tropomyosin– troponin states, determined by pyrene–tropomyosin ﬂuorescence potx

... tropomyosin and actin, the IANBD probe retained its typicalresponses to changes in Ca2+ and S1 binding (Fig. 6).Fitting binding curves to obtain binding parametersis difﬁcult in the case of Ca2+because ... the measuredeffects of Ca2+on binding in the presence of ATP[35,36], equilibrium binding in the presence of ADP[16], binding kinetics [15,37] and the general features of ATPase activities ... k2bq2zij9>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>=>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>;ð2Þp1 and p2are fractions of actin units in the inactive and active states, respectively; hI and h2are fractions of actincontaining bound S1 in the inactive and active states,respectively;...

Báo cáo khoa học: Membrane distribution of epidermal growth factor receptors in cells expressing different gangliosides doc

... (pseudo-coloured green). C, GandKaremergedimagesfromAandB,EandFandIandJ,respectively.AnenlargementoftheboxedareasinC,GandKareshowninD,HandL, respectively. Images shown in this ﬁgure are single xy confocal ... microdomains (GEM) of the plasma membrane and to be involved in the regulation of epidermal growthfactor receptor (EGFr or ErbB1) activity. To gain furtherinsight into the mechanisms involved in ... X-100,indicating that it is mainly excluded from GEM. Interest-ingly, the behaviour of the receptor to extraction with coldTriton X-100 was independent of its activation state becausebinding of...

Báo cáo khoa học: " THE DISTRIBUTION OF WORD LENGTH IN TECHNICAL RUSSIAN" pdf

... by a machine and they are of limited variety, so that their listing in a dictionary is practicable. From the practical point of view, the distri-bution is useful in planning input and storage ... not shared by the others, of being concentrated at relatively low values of length, and of having no elements exceeding a certain length (Fig. 1). Words, defined in this fashion, can readily ... theoretical interest of this distribution arises from the possibility of using it as a basis for an operational definition of words in printed texts. If texts are considered purely as sequences of...

Báo cáo khoa học: Crystal structures of bovine odorant-binding protein in complex with odorant molecules doc

... 1).ResultsBinding of AMA and odorants in solutionThe dissociation c onstants of the AMA–OBP c omplexes fo rthe bovine and porcine OBP isoforms were, respectively, 1 .0 and 1.5 lM and the corresponding ... onformational changesinvolving protein loops or backbone. T hus, the role of OBP in signal triggering remains unresolved.3Keywords: crystal str ucture; domain swapping; odorant-binding protein; olfaction.Odorant-binding ... 2-iso-butyl-3-metoxypyrazine and (E) d ihydrom yrcenol. Each ﬂuorescencepoint on the y-axis s hows the concentration of AMA still b ound per monomer of bovine odorant- binding protein (bOBP) and p o rcine odorant-binding...

Tài liệu Báo cáo khoa học: PCR detection of nearly any dengue virus strain using a highly sensitive primer ‘cocktail’ ppt

... strains of Japaneseencephalitis virus, 28 strains of St Louis encephalitis virus, 172 strains of West Nile virus and 24 strains of yellow fever virus using BLASTn [22]. The genome sequences of theflaviviruses ... todengue virus when computationally tested against 291strains of other nondengue flaviviruses, including strains of Japanese encephalitis virus, St. Louis encephalitis virus, West Nile virus and yellow ... presence of virus [14].Nucleic acid-based assays offer rapid and specificdetection and serotyping of dengue virus, and aregradually replacing serological and culture techniques.These methods include...

Tài liệu Báo cáo khoa học: Down-regulation of reduced folate carrier may result in folate malabsorption across intestinal brush border membrane during experimental alcoholism docx

... counterstaining.Haematoxylin–eosin stainingHaematoxylin–eosin staining was carried out followingthe routine histological method described by Kayser and Bubenzer [38]. The haematoxylin–eosin staining techniqueemploys ... KH2PO4 and 8 mm Na2HPO4, and kept at 37 °C for 15 min in abeaker containing NaCl ⁄ Pi. The intestine was then ﬁlledwith a solution containing 1.5 mm EDTA and 0.5 mmIntestinal folate ... absorptive site of folic acid, i.e. the smallintestine.Expression of the RFC protein in BBM of theintestineThe effect of chronic alcoholism on the level of expres-sion of the RFC protein at the...

Tài liệu Báo cáo khoa học: Identiﬁcation of a novel matrix protein contained in a protein aggregate associated with collagen in ﬁsh otoliths pdf

... determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. SixGST-fused recombinant proteins containing the three distinctivedomains of tandem repeat 1 ... Of these recombinantproteins, rOMM-64-I, III, IV and V, which include theGlu-rich domain, were found to have calcium-bindingactivity. rOMM-64-II and -C and GST were stainedred using ‘Stains-all’ ... band was obtained after digestionFig. 3.45Ca overlay analysis of fusions of GST and recombinantOMM-64 variants (rOMM-64-I-V and -C), containing differentdomains of the protein, to determine...

Tài liệu Báo cáo khoa học: The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases pptx

... DNA and AdoHcy(AdoMet) [44], M.HhaIconsists of two domains, the large domain containingthe S-adenosyl-l-methionine (AdoMet) binding site and the catalytic center, and the small domain contain-ing ... CGM and GCX+⁄ CGM), 5-methylcytosine was introducedinto one of the strands of the recognition site instead of the target cytosine. The melting curves of the hemi-methylated X+CG ⁄ CGM and ... Thus, the stereochemistry of the B[a]P-N2-dG adducts in DNA does not in uenceDNA binding in the case of M.SssI, but, in contrast,does affect DNA binding in the case of M.HhaI.The (+)-trans-B[a]P-N2-dG...

Tài liệu Báo cáo khoa học: The localization of FGFR3 mutations causing thanatophoric dysplasia type I differentially affects phosphorylation, processing and ubiquitylation of the receptor pptx

... staining of 293-VnR and ATDC5 cells expressing the Y373C mutant with anti-FGFR3 and anti-phosphotyrosine sera showed bothintracellular and cell-surface phosphotyrosine staining(Figs 2Eb,c and ... Conversion of Lys650 into methionine in the TK2 domain induceshyperphosphorylation and marked intracellular retent-ion of the mutant receptor leading to phosphorylation of target signalling molecules ... phosphorylation of proteinsinvolved in the trafﬁcking of the receptor, includingc-Cbl, could account for its intracellular retention. Bycontrast, TDI mutations in the ECD or disruption of the termination...

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