Báo cáo khoa học: "Mechanism of UV light-induced photorelaxation in isolated rat aorta" pdf
Báo cáo khoa học: "Mechanism of UV light-induced photorelaxation in isolated rat aorta" pdf
... on the UV light-induced photorelaxation of rataortas. Fig. 4. Inhibitory effect of methylene blue (MB, 10 uM) on UV light-induced photorelaxation of rat aorta.Fig. 5. Tracing of the inhibitory ... thecharacteristic of UV light-induced photorelaxation, theadministration of NOS inhibitor, L-NAME, its derivative,D-NAME, and NO donor, L-arginine, significantlyinduced the magnitude of potentiation of photorelaxation on ... Mechanism of UV light-induced photorelaxation in isolated rat aorta 83Effect of Ca2+-free and verapamil containing mediumon UV light-induced photorelaxation As it has been...
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Báo cáo khoa học: Regulation of matrix metalloproteinase activity in health and disease pdf
... processing of one or several nuclear proteins, whereas the other isindependent of the processing capacity of the protein-ase and involves the HPX domain. A DNA-bindingdomain was found in the ... integrin-binding ligandN-linked glycoprotein (SIBLING)Individual members of the SIBLING family are knownto bind strongly to both pro- and active forms of specific MMPs. Bone sialoprotein binds ... autocatalytic step of the activation by binding tothe 64 kDa intermediate form of MMP-2 [52]. Bindingand activation of MMP-2 was abrogated in the pres-ence of avb3 integrin-binding macromolecules...
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Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx
... DHNA inhibitors. Thebinding of the inhibitors to the enzymes cause adecrease in their fluorescence intensities. The increasingfluorescence intensities in Fig. 5A were obtained bysubtracting ... set of control data was obtained in theabsence of the protein. The data set obtained in the absence of the protein was then subtracted from the correspondingdata set obtained in the presence of ... measured the binding of HP in theabsence of GA. NP and MP are the oxidized forms of the pterins, with a double bond between C7 and N8instead of a single bond as in the reduced pterins(DHNP and...
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Tài liệu Báo cáo khoa học: Mechanism of protection of peroxidase activity by oscillatory dynamics docx
... 2003 of melatonin, as illustrated in Fig. 3. Figure 3A shows timeseries of the total enzyme concentration in the presence of different concentrations of melatonin, while Fig. 3B showsa plot of ... [34]. Increasing theconcentration of 4-hydroxybenzoic acid also resulted in adecrease in the rate of enzyme inactivation. However, whenthe reaction is in a stationary state, the concentration of ... essentially independent of theconcentration of the cofactor. The difference in steady stateand mean oscillatory concentrations is more pronouncedwith increasing cofactor concentration because...
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Báo cáo khoa học: Mechanism of the reaction catalyzed by dehydroascorbate reductase from spinach chloroplasts doc
... concentra-tion of one substrate at various fixed concentrations of the other sub-strate. (A) Reciprocals of initial rates of reduction of DHA are plottedagainst the reciprocals of concentrations of DHA ... replotted against the reciprocals of the con-centrations of GSH. (B) Reciprocals of initial rates of reduction of DHA were plotted against the reciprocals of concentrations of GSH atfixed concentrations ... first-binding molecule of GSH; KGSH2m, Kmfor the second-binding molecule of GSH;KDHAi, inhibition constant for DHA; KGSH1i, inhibition constant forthe first-binding molecule of GSH;...
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Báo cáo khoa học: Mechanism of mild acid hydrolysis of galactan polysaccharides with highly ordered disaccharide repeats leading to a complete series of exclusively odd-numbered oligosaccharides doc
... gas, at a flow rate of 1.0 mLÆmin)1. The oven temperature was initially keptat 50 °C for 4 min, then increased to 250 °C at a rate of 8 °CÆmin)1, and finally kept at 250 °C for 10 min. The ionsource ... Guangling Jiao1, Sumei Ren1and Wengang Chai1,21 Glycoscience and Glycoengineering Laboratory, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China2 Glycosciences Laboratory, ... CH3CN containing0.01 m TFA, at a flow rate of 0.6 mL Æ min)1. Detection wasby UV (280 nm) and ELSDs in series. The former was forthe detection of 5-HMF, and the latter for the detection of 3,6-anGal.The...
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Báo cáo khoa học: Mechanism of the ring contraction process in vitamin B12 biosynthesis by the anaerobe Propionibacterium shermanii under aerobic conditions docx
... owing to18O a-isotope effect in hertz.fRatio is the18O-retention ratio of 13C18O-amide oxygen in 13C18O-vitamin B12(5). These ratioswere calculated from a comparison of the intensities ... migra-tion of ring D followed by cleavage of the carbon–oxy-gen bond of the c -lactone at C1 of 13C,18O-precorrin-3x [8–10] as shown in the lower row of Fig. 1.We were interested in the mechanism ... acetate groupat C1, and migration of ring D by cleavage of the car-bon–oxygen bond at C1, observed in P. denitrificans,do not operate in P. shermanii even in the presence of oxygen.Experimental...
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Tài liệu Báo cáo khoa học: Roles of heat shock factors in gametogenesis and development pptx
... effect of HSF4 onchromatin. In the absence of HSF4, histone H3K9methylation is induced and HSF1 binding is reduced,indicating that HSF4 facilitates HSF1 binding viachromatin remodelling. Heat ... section and scheme of the coronal section at E14.5 in (F). (A) At E11.5, HSF2 is expressed in forebrain, midbrain and in hindbrain except for the midbrain, hindbrain midline. Surprisingly, the HSF2 ... cur-rently performed to investigate the impact of incorrectneuronal migration in different parts of the Hsf2) ⁄ )brains.A genetic basis for differences in mouse strains in eliciting the HSR has been...
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Tài liệu Báo cáo khoa học: Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses pdf
... solution wasincreased linearly by approximately 1.0 °CÆmin)1. Thermaldenaturation of these proteins was reversible in the presence of 1 m GdnHCl. The temperature of the midpoint of thetransition, ... because of the intrinsic flexibility of theloop containing His124 and the crystal packing effect.Destabilization mechanism of So-RNase HIA combination of the six thermostabilizing mutationsincreases ... structure of the 6·-RNase HI protein was deter-mined at 2.5 A˚resolution. The main chain fold and interactions of theside-chains of the 6·-RNase HI protein were basically identical to those of the...
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Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
... fosfomycin binding due to charge–chargerepulsions. Most interestingly, the uncharged valine sidechain completely abolishes fosfomycin binding in theabsence of UDPNAG. Clearly, UDPNAG binding createsadditional ... Lysine 22 and arginine 120 in MurA (Eur. J. Biochem. 271) 2685the positively charged side chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin ... enolpyr-uvyl-accepting substrate, shikimate-3-phosphate, does notinteract with R124 in the ternary complex [20]. Hence, themechanism of binding of fosfomycin by reinforcement of aninitial binding site (mainly K22...
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