... Biol. 248, 385±401.10. Sato, A ., K oyama, S., Yamada, H., Suzuki, S., T amura, K.,Kobayashi, M., Niwa, M., Yasuda, T., Kyogoku, Y. & Kobay-ashi, Y. (2000) Three-dimensional solution structure ... were activated by injecting 35 lL N-ethyl-N¢-[(dimethylamino)propyl]carbodiimide/ N-hydroxysucci-nimide a t 5 lLámin)1. Subsequently, rhIGF1R was coupledto the CM5 se nsor chip by injecting ... a commercial[3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTT)-based] assay (CellTiter, 96Ò, Aqueous One Solution Assay; Promega).Absorbances were...