Báo cáo khoa học: "the Role of Non-Timber Forest Products (NTFPs) in Livelihood Strategies and Household Economies in a Remote Upland Village in the Upper Ca River Basin, Nghe An, Vietnam" pdf

Tài liệu Báo cáo khoa học: Key role of the loop connecting the two beta strands of mussel defensin in its antimicrobial activity docx

... membrane. The way this contacttakes place and the molecular features of the proteininvolved are yet to be deciphered. The lack of informationabout the mode of action and the availability of a ... (i.e. the b-hairpin loop and part of the adjoining b-sheet) and Ôresidues in the loop connecting b-strand and a- helix and contiguous residues on the a- helix and the last part of b-strand 3Õ [35]. ... compared with results obtained by other groupswho also point to the role of the connecting loop of the b-hairpin of defensins. A combination of mutationalanalysis [35] and structural analysis of...

Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf

... proteases(Fig. 1). The catalytic triad residues Asp113, His57, and Ser194 in API are placed at an identical location to those of chymotrypsin and bovine trypsin. The catalytic triadresidues and ... typically W169V and W16 9A, deviates from the original position. In the struc-tural deviation, the solvent ASA of the side-chain of His210increased with the decrease in size of the side-chain atresidue ... in the role of the electrostatic properties in enzymatic catalysis of API, and in distinguishing the functionally catalytic quadruple Ser194–His57–Asp113–His210 from the usual catalytic triad...

Báo cáo khoa học: Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast pptx

... (5¢-atagacacgcaaacacaaatacacacactaaattaataatgaccggatcATGTACTTCCCCTTTTTAGGCAGAT-3¢) and lip2 (5¢-cagtagagacatgggagatcccccgcggaattcgagctcggtacccgggTCATTCTTTATTTAGAGCATCCAGC-3¢). The sequences in lower-case ... wasgenerated by disrupting the ATF2 gene of CA1 0 with the KanMX4 cassette, which confers G418 resistance. Primers5¢ATF2-Kan 5¢-AGACTTTCAAACGAATAATAACTTCAGCAATAAAAATTGTCCAGGTTAATtccagcgacatggaggccc-3¢ ... 5¢-AGACTTTCAAACGAATAATAACTTCAGCAATAAAAATTGTCCAGGTTAATtccagcgacatggaggccc-3¢ and 3¢ATF2-Kan: 5¢-TTGTACGAGCTCGGCCGAGCTATACGAAGGCCCGCTACGGCAGTATCGCAcattcacatacgattgacgc-3¢ (nucleotides in lower case arespeciﬁc to the KanMX4 module) were used for PCR withpFA6-MX4 as...

Báo cáo khoa học: Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure pdf

... efﬁciency, indicating that the last resi-due, Leu559, has a role in the catalysis of ABTS oxida-tion. The catalytic constants for the phenolic substrates2,6-DMP and syringaldazine were not greatly affectedby ... suggested that the secondary structure of the mutant was not signiﬁcantlyaltered. Even a single amino acid change in the C-terminus of MaL (L55 9A) changed the catalyticproperties of the laccase. The ... laccase.Our structural analysis of wild-type MaL indicatedthat the last four amino acids of the mature proteinpenetrate the tunnel leading from the surface to the trinuclear site and form a...

Báo cáo khoa học: Dual role of Nbs1 in the ataxia telangiectasia mutateddependent DNA damage response ppt

... contains a forkhead-associated (FHA) domain and a Brca1 COOH-terminus (BRCT) domain in itsNH2-terminal region, as well as an Mre11-bindingdomain and an ATM-binding domain in its COOH-terminal ... of DNA strand breakage and in the activation of the ATM-dependent DNA damage signaling path-way [6,34–36]. However, normal activation of the kin-ase activity of ATM and phosphorylation of the ... Nbs1 acts both as a downstream target of ataxia telangiectasia mutated in the S-phase checkpoint of the cell cycle aswell as an upstream modulator or activator of ataxia telangiectasiamutated in...

Báo cáo khoa học: Functional role of the linker region in puriﬁed human P-glycoprotein pot

... basal and substrate-stimulated ATPase activity than native P-gp(Fig. 2 and Table 2). These results indicate that, as for the native P-gp, the N-half and the C-half fragments of the linker-cleaved ... proteases at 20 °C for 1.5 h. The cleavage was stopped and ATPase activity was measured as indicated in the Materials and methods. All data represent the means ± SD from three independentassays.Transport ... 2470 a Values in parentheses indicate the relative molecular mass of each transport substrate.bkbasalis the kcatvalue for basal ATPase activitywith no transport substrates.cksubis the kcatvalue...

Báo cáo khoa học: Functional role of Bb-chain N-terminal fragment in the ﬁbrin polymerization process pdf

... in the N-terminal region of the Aa-chain by the splitting off of fibrinopeptide A [6]. Site a is formed by amino acid residues cGln329,cAsp330, cHis340 and cAsp364, situated in the cC-domain ... in the fibrinbC-domain [7]. The ‘B’–‘b’ pairing leads to structuralrearrangements in the D-domain and the strengthening of protofibril lateral contacts [9,10].Many indirect data show that other ... for arginine at Bb14. Turbidityanalysis showed an increase in lag-time and a decrease in maximum polymerization rate of this fibrinogen in the desAA fibrin form. The final turbidity of thesefibrin...

Báo cáo khoa học: "the Role of Non-Timber Forest Products (NTFPs) in Livelihood Strategies and Household Economies in a Remote Upland Village in the Upper Ca River Basin, Nghe An, Vietnam" pdf

... UNIVERSITY OF AGRICULTURE The Role of Non-Timber Forest Products (NTFPs) in Livelihood Strategies and Household Economies in a Remote Upland Village in the Upper Ca River Basin, Nghe An, Vietnam ... gain cash income from timber and agricultural products. Livelihood Strategies of Households in Tha Lang Hamlet In this analysis, livestock raising was chosen as a key indicator of the intensification ... this village in the remote upland region of the upper Ca river basin, Nghe An, Vietnam. Our research found that after stopping opium cultivation, the village s farmers have primarily practiced...

Báo cáo khoa học: Biological role of bacterial inclusion bodies: a model for amyloid aggregation potx

... software such as aggrescan [42] or tango [43].Protein aggregation can be understood as an anoma-lous type of protein–protein interaction. As for nativeinteractions, the attainment of ordered aggregatedstructures ... use of these underesti-mated bacterial aggregates as intriguing models for the analysis of protein–protein interactions in the context of amyloid and prion diseases.Dynamics of IB formation and biological ... dis-position in these aggregates, in particular in the case of HET-s PFD where the NMR signals of the two types of aggregates overlap signiﬁcantly [61,62,65]. Overall,it appears that the formation of amyloid-like...

Báo cáo khoa học: Dual role of oxygen during lipoxygenase reactions potx

... 2523 of the hydrocarbon chain, via epoxidation or dimeriza-tion [24]. (b) Escape of the catalytically inactive ferrousLOX from the catalytic cycle. When the catalyticallyactive ferric LOX catalyses ... the absorbance at 275 nm during the time course of the reaction. From Fig. 6Aa it can beseen that there was a linear increase in absorbance at275 nm and subsequent HPLC analysis indicated the formation ... For any time point of the progresscurve, the value of the variable monitored was calculated as the arithmetic mean taken over the values of the 10 pre-ceeding and 10 succeeding data points. Initial...

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