... the
flow path above the column improves peak resolution in two
76
7
Chapter 7
Mobile phase
pumps and
degassers
78
In food analysis, pump performance is critical. In the
examples, we describe a low-pressure ... perform
according to food analytical requirements. The former has a
single dual-piston mechanism for low-pressure gradient
formation, whereas the latter has a double dual-...
... . 76
Gradient formation at high pressure . . . . . . . . . . . . . . . 77
Gradient formation at low pressure . . . . . . . . . . . . . . . 77
VII
Part Two
The Equipment Basics
HPLC
for Food
Analysis
A ... used for various purposes in modern food processing.
For example, citric acid adds a fresh, acidic flavor, whereas
succinic acid gives food a more salty, bitter taste....
...
array
detector
Chromatographic conditions
HPLC and UV-visible diode-array detection have been
applied in the analysis of antioxidants in chewing gum.
Spectral information and retention times were used for
identification.
5
Sample ... conditions
HPLC and UV-visible diode-array detection have been
applied in the analysis of preservatives in white wine and
salad dressing. Spectral inf...
... carbon,
Working potential: 1 V
Operation mode: amperometry
Chromographic conditions for electrochemical detection
The HPLC method presented here was used for the
analysis of iodide in table salt.
17
34
HPLC ... conditions
The HPLC method presented here was used for the analysis
of anions in drinking water.
Figure 23
Analysis of anions in drinking water with indirect UV-det...
... have set maximum
tolerance levels for these compounds in foodstuffs. HPLC is
now preferred for the analysis of amines in food matrices
because of its shorter analysis time and relatively simple
sample ... software
components needed
for successful HPLC,
and an introduction to
the analytical techniques
that have become
routine in food analysis
Part Two
Chromatographic con...
... 45
shows the advantage of Peltier control over conventional
air cooling.
60
70 .40
70 .60
70 .80
71 .00
71 .20
123
45
67
8
910
Run number
Retention time
Day
Night
Day
Conventional
column oven
Agilent ... prerequisite for successful
food analysis. The broad selection of cleanup and
enrichment techniques takes into account the many
matrices and compound classes under study.
Sampl...
... 13 .72 A 19.024 18.9 87 19.000 1000 1000 1 .7
N-Acetylsulfapyidine 14.66 A 23.3 07 23.282 23.282 976 1000 1.1
Ethopabat *up 13.40 A 23. 874 23.840 23.848 911 996 2.3
Benzthiazuron 12.80 A 24.0 47 24.024 ... is a well-established
technique for food analysis, LC/MS is only now emerging as
a useful tool in this area. A GC-based analysis is appropriate
only for those food compounds...
... [cis]-9)
molecular weight = 884 .78 1
molecular formula = C
57
H
104
O
6
8
APCI requires some compound volatility and is less suitable
for highly thermally labile compounds. Figure 74 shows a
typical triglyceride ... provide for automated drawing of the baselines
during postrun replotting and for the plotting of calibration
curves showing detector response. For unattended analyses...