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Holcim has strengthened its balance sheet and earning power and positioned itself as an attractive group in the international capital markets

Holcim has strengthened its balance sheet and earning power and positioned itself as an attractive group in the international capital markets

Holcim has strengthened its balance sheet and earning power and positioned itself as an attractive group in the international capital markets

... plant and equipment 36 83 113Purchase of financial assets, intangible and other assets 36 (2,153) (1,014)Disposal of financial assets, intangible and other assets 36 874 572Cash flow used in ... dividend and interest income on financial assets, depreciation on non-operating assets and other net income, which includes profits and losses of associated companies, profits and losses from the ... increasecarried out in the first half of 2004. The cash inflow amount-ing to CHF 1,456 million was used to finance the buyouts ofminority interests in Mexico and the Philippines and underpinthe...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

... °C.a-L-1,3-Arabinofuranosidase activity in the GALA pre-paration was measured as described before [27], using 10 and 100 lL of the enzyme preparation (in duplicate) and incubation times of 1 and 4 h.Analytical ... usingazo-galactan. Transformant NW290::pIM3982.77 wasselected as the highest b-1,4-endogalactanase producingtransformant and was used for the purification of GALA.Growth of this transformant ... [33]. The fusion construct (pIM3982) was made by introducing an NsiI site at the translation start point of galA and by thencloning a fragment, starting at this NsiI site and containing the galA...
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Báo cáo khoa học: Purification and characterization of zebrafish hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot

Báo cáo khoa học: Purification and characterization of zebrafish hatching enzyme – an evolutionary aspect of the mechanism of egg envelope digestion pot

... PAGE of the MHCE digest after a 10 and 40 min incubation gave two bands (43 and 39 kDa), and an additional band (36.5 kDa) was observed aftera 120 min incubation (Fig. 8A, lanes 3–5). These corre-sponded ... bands with molecu-lar masses of 43, 39 and 36.5 kDa were observed(Fig. 5, lane 2). After further incubation (60 min), the 39 kDa band disappeared, and only two bands with the same mobility as ... 90 min. The 43 kDa bandbecame weaker than that of the 50 min incubation,suggesting further digestion of the 43 kDa product.Furthermore, their 36.5 kDa bands became broaderthan that for the...
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Báo cáo khoa học: Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursor doc

Báo cáo khoa học: Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursor doc

... and androstadienolbiosynthesis In order to determine the effect of cyt b5 in P450c1716-ene-synthase and 17a-hydroxylase/17,20-lyase activities,we performed transfection assays with increasing ... increasing amountsof DNA fragments encoding cyt b5 and monitored the formation of androstadienol and DHEA. As shown in Fig. 5 the stimulation of DHEA and androstadienolproduction from preg increases ... through its 17a-hydroxy-lase activity and then transforms this intermediate intoDHEA through its 17,20-lyase activity. In order to deter-mine whether the transformation of preg into androstadie-nol...
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Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx

Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx

... oxidizing conditions. (c) Apo-ACP (O) and holo-ACP (h) in each fraction was quantified by measuring the intensity of the silver-stained protein bands usingQUANTITY ONE software and plotted against ... hydrochloride-induced denaturation, where the observed CD signal ateach point in the unfolding experiment is mobs, wasanalyzed with the equationwhere No and Dorepresent the intercept and aN and ... an- hydrase (29 kDa) and BSA (66 kDa), were separated and fractionated under the same conditions and detected bySDS ⁄ PAGE and silver staining. The protein bands werequantified by quantity one (BioRad,...
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Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf

Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf

... Cytokine-inducible SH2 protein-3 (CIS3 ⁄ SOCS3)inhibits Janus tyrosine kinase by binding through the N-terminal kinase inhibitory region as well as SH2domain. Genes Cells 4, 339–351.4 Sasaki A, Yasukawa ... the kinase inhibitory region and the entire extended SH2subdomain form a single a-helix. The mapping of the true SH2 domain, and the location of its C terminus more than 50 residues further ... resonances in the random coil region of the 15N-HSQC spectrum and showed significantly narrower line-widths than anyother residues in the protein. This indicates that the PEST sequence is an unstructured,...
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Báo cáo khoa học: Apolipoproteins A-I and A-II are potentially important effectors of innate immunity in the teleost fish Cyprinus carpio pot

Báo cáo khoa học: Apolipoproteins A-I and A-II are potentially important effectors of innate immunity in the teleost fish Cyprinus carpio pot

... (b and a), an intermedia ryfragment (band b) and a more stable third band (band c)were recognized by the specific antiserum against carpapoA-I. However, when incubated with the antiserumagainst ... use in antimicrobialassays, p rotein concentration of apolipoprotein samples wasdetermined by the b icinconinic acid method [17] and its purity and integrity was checked by S DS/PAGE accordingto ... (apoA-I and a poA-II) and corresponds to the mostabundant plasma protein in sever al teleost fish [9,10], witha concentration as high as 1 gÆdL)1 in the carp [11].Although the main role of HDL and...
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Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot

Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot

... that the deadenylation machinery is pre-existing in the cells and deadenylates IFN-b mRNA as soon as its synthesis isinduced by stimulating agents. The fact that the 65-kDaprotein binds ARE and ... and were incubated for 6 h before transfection.After transfection, the cells were incubated for another 24 h and then infected by Sendaı¨virus for 2 h. Cells were washed and preincubated in ... 1A). We then analysed, by Northern blot, the induc-tion and decay of IFN-b transcript in the same conditions.AsshowninFig.1B,IFN-b mRNA was detectable 4 hafter the beginning of the infection,...
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Báo cáo khoa học: An intermediate step in the evolution of ATPases ) the F1F0-ATPase from Acetobacterium woodii contains F-type and V-type rotor subunits and is capable of ATP synthesis Michael Fritz and Volker Muller ¨ docx

Báo cáo khoa học: An intermediate step in the evolution of ATPases ) the F1F0-ATPase from Acetobacterium woodii contains F-type and V-type rotor subunits and is capable of ATP synthesis Michael Fritz and Volker Muller ¨ docx

... disrupt the c oligomer and blotted against c1antibodies. Lane 4: ATP synthase was incubated for 10 min at 80 °C, and blottedagainst c1antibodies. Lane 5: the sample was incubated for 10 min at ... synthase c subunits, they are predicted to span the membrane like a hairpin and to have one ion-bind-ing site. In contrast, subunit c1is similar to the c sub-units of V1V0-ATPases and ... woodii The specific ATPase activity of the complete enzymewas in the range 5–9.9 UÆmg)1protein, depending on the batch. This is in the same range as the one deter-mined previously with the incomplete...
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