... strains PP30[pHSC8 2b] , PP30[pHSP82], PP30[hHsp90a], and< /b> PP30[hHsp9 0b] . Ten micrograms of total soluble protein was gelfractionated, and < /b> then western blotted; the < /b> blot was then probedwith anti-(Achlya ... ‘bait’ fusions comprising Hsp90a and< /b> Hsp9 0b with C-terminal BD extensions (Hsp90a-BD,Hsp9 0b- BD; see Experimental procedures). These werethen mated to cells expressing the < /b> previously describedGal4p ... In the < /b> absence of ERK5 expres-sion, expression levels were less than 2 mU.A B Fig. 5. (A) The < /b> relative strengths of Hsp90a-BD–AD-Slt2p, Hsp9 0b- BD–AD-Slt2p, Hsp90a-BD–AD-ERK5 and < /b> Hsp9 0b- BD–AD–ERK5Y2H...