... [40],RmaxðtheoreticalÞ¼ðMr;analyte=Mr;ligandÞnðAÞRimmobilizedð1Þwhere Mr,analyte and Mr,ligandare the relative molecular mass of the analyte and ligand, respectively; n is the number of analyte-binding sites on the protein; (A) is the fraction of active ... of the equilibrium-binding isotherms obtained in the absence of ATP ⁄ Ca2+(d), in the presence of 500 lM ATP (.) and in the pres-ence of 500 lM Ca2+(s) in the flow buffer.Grp94–ligand interactions ... and a negative DRU due to the ligand-induced conform-ational change, no exact value for the binding affinity and the stoichiometry of ATP binding could be deter-mined. By contrast, in the...