Introduction to Modern Liquid Chromatography, Third Edition part 14 pps
Introduction to Modern Liquid Chromatography, Third Edition part 14 pps
... 1303.8.5 Report Generation, 1303.8.6 Regulatory Functions, 1303.9 EXTRA-COLUMN EFFECTS, 131 Introduction to Modern Liquid Chromatography, Third Edition, by Lloyd R. Snyder,Joseph J. Kirkland, ... the pump. The frit acts as a weight to keep the inlet tubing at thebottom of the reservoir, but its primary function is to provide backup filtration to remove particulate matter, such as dust, ... from a reservoir into a pump, which controls the flow rate andgenerates sufficient pressure to drive the mobile phase through the column. Aninjector or autosampler is used to place the sample...
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Introduction to Modern Liquid Chromatography, Third Edition part 3 ppsx
... 65113.10.3 Liquid- Chromatography Modes for PolymerAnalysis, 65313.10.3.1 Size-Exclusion Chromatography, 65313.10.3.2 Interactive Liquid Chromatography, 65313.10.3.3 Liquid Chromatography underCritical ... Anion-Exchange Chromatography, 61913.5.2 Reversed-Phase Chromatography, 620xxiv CONTENTS 14. 6 Chiral Stationary Phases and Their Characteristics, 681 14. 6.1 Polysaccharide-Based CSPs, 682 14. 6.2 Synthetic-Polymer ... CSPs, 689 14. 6.3 Protein Phases, 691 14. 6.4 Cyclodextrin-Based CSPs, 697 14. 6.5 Macrocyclic Antibiotic CSPs, 699 14. 6.6 Chiral Crown-Ether CSPs, 706 14. 6.7 Donor-Acceptor Phases, 707 14. 6.8 Chiral...
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Introduction to Modern Liquid Chromatography, Third Edition part 6 pps
... the early days of HPLC the technique was sometimes referred to as ‘‘high-pressure liquid chromatography’’ or ‘‘high-speed liquid chromatography, ’for reasons suggested by Figure 1.5g. Figure 1.5h ... liquid liquid partition chromatography that waslater improved in various ways. HPLC with a liquid stationary phase was sincereplaced by bonded-phase HPLC, the use of CCC as an alternative to ... Journal of Chromatographic Science, Preston• Journal of Chromatography A, Elsevier• Journal of Chromatography B, Elsevier• Journal of Liquid Chromatography, Wiley• Journal of Separation Science,...
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Introduction to Modern Liquid Chromatography, Third Edition part 13 ppsx
... Chromatogr. A, 1119 (2006) 140 .22. J. C. Giddings, Dynamics of Chromatography, Part 1, Principles and Theory, Dekker,New York, 1965.23. G. Guiochon, in High-Performance Liquid Chromatography. ... Snyder and J. J. Kirkland, Introduction to Modern Liquid Chromatography, 2nded., Wiley-Interscience, New York, 1979, pp. 34–36.46. Reviewer Guidance. Validation of Chromatographic Methods, Center ... W. Carr, in High Performance Liquid Chromatography,P.R.Brownand R. A. Hartwick, eds., Wiley-Interscience, New York, 1989, p. 1.25. J. H. Knox, in Advances in Chromatography, Vol. 38, P. R. Brown...
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Introduction to Modern Liquid Chromatography, Third Edition part 33 pps
... different samples andchromatographic modes; this topic is addressed below and in individual chaptersthat focus on sample type and/or chromatographic mode (Chapters 6–9, 13, and 14) . Finally, when the ... 6 .14, compared to the Symmetry column, values of Fs(−C) are equal to 26 for theAlltima column, 12 for the Luna column, and 16 for the Spherisorb column. Avalue of Fs(−C)>5 can lead to ... developed, it is likely to be used over an extended period and in differentlaboratories. Columns degrade during routine use and must therefore be replacedfrom time to time. As discussed in...
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Introduction to Modern Liquid Chromatography, Third Edition part 34 ppsx
... in flow ratefrom 2.0 to 1.0 mL/min (not shown) results in an insignificant increase in resolution (to Rs= 1.2), while run time is doubled to 10 min. Alternatively, the particle sizecan be reduced, ... Sample and con-ditions as in Figure 6 .14, except as indicated in figure. Chromatograms recreated from data of[8, 9, 69].form a single column. Two alternatives to the latter procedure have been suggested,neither ... (called‘‘phase-optimized liquid chromatography’’ or POPLC®from Bischoff). Experimentswith individual columns define sample retention for each column type, and it is thenpossible to predict retention...
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Introduction to Modern Liquid Chromatography, Third Edition part 40 pps
... the analysis of any mixture that contained organic acids or bases. Togetherwith liquid liquid partition and adsorption chromatography, IEC then accountedfor most of the reported separations ... CHROMATOGRAPHY (IEC)Ion-exchange chromatography (IEC) is an important separation technique, but forthe most part, today, with limited applications:• mixtures of inorganic ions (ion chromatography)• ... ION-EXCHANGE CHROMATOGRAPHY (IEC) 351samples that contain too many components to be separated in a single HPLC run.The principle of operation is the use of a first HPLC separation to achieve partialseparation...
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Introduction to Modern Liquid Chromatography, Third Edition part 41 pps
... chromatography(NPC). Another term used to describe NPC is adsorption chromatography,inrecognition of the fact that retained solute molecules are attached to (or adsorbedonto) the surface of particles ... standards by anion-exchange chro-matography with amperometric detection. Sample: 1, myo-inositol; 2,D-sorbitol; 3, lactitol;4,L-fructose; 5, rhamnose; 6, D-galactose; 7, D-glucosamine; 8, D-glugose; ... Chromatogr. Sci. , 38 (2000) 386.34. S. Pous-Torres, J. R. Torres-Lapasi´o, J. J. Baeza-Baeza, and M. C. Garc´ıa-´Alvarez-Coque,J, Chromatogr. A, 1163 (2007) 49.35. S. Pous-Torres, J. R. Torres-...
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Introduction to Modern Liquid Chromatography, Third Edition part 43 pps
... affected by which isomer can better position itself adjacent to thesurface, so as to allow each polar methoxy group in the molecule to interactwith an adjacent silanol group on the surface. In ... provided in Figure 8 .14 for the separation of five isomers of the com-pound retinol. In the RPC separation of Figure 8.14a, there is little separation of382 NORMAL-PHASE CHROMATOGRAPHYCyano columnDiol ... silicacolumns—compared to polar-bonded-phase columns. Consequently a change fromsilica to a polar-bonded-phase column, with further variation of the mobile phase(change in %B and/or B-solvent), is unlikely to...
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Introduction to Modern Liquid Chromatography, Third Edition part 45 pps
... Chromatography (NPC), 4669.5.3 Hydrophilic Interaction Chromatography (HILIC), 4679.5.4 Ion-Exchange Chromatography (IEC), 4709.6 PROBLEMS, 470 Introduction to Modern Liquid Chromatography, Third ... 1999,http://www.lschrom.com.21. M. Z. Kagan, J. Chromatogr. A, 918 (2001) 293.22. L. R. Snyder and J. J. Kirkland, Introduction to Modern Liquid Chromatography, 2ndedn., Wiley-Interscience, New York, ... Chromatogr. A, 1064 (2005) 85.33. L. R. Snyder, J. Chromatogr. Sci., 21 (1983) 65.34. V. R. Meyer, J. Chromatogr. A, 768 (1997) 315.35. P. Jandera, J. Chromatogr. A, 965 (2002) 239.36. R. W. Stout,...
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