... W. Stout, J. J. Destefano, and L. R. Snyder, J. Chromatogr., 282 (19 83) 2 63.
34 . J. H. Knox and H. P. Scott, J. Chromatogr., 282 (19 83) 297.
35 . K. Miyabe, J. Chromatogr. A, 1167 (2007) 161.
36 . ... Snyder and J. J. Kirkland, Introduction to Modern Liquid Chromatography, 2nd
ed., Wiley-Interscience, New York, 1979, pp. 34 36 .
46. Reviewer Guidance. Validation of Chromatog...
... min
30
o
C; k* ≈ 5, R
s
= 0.1
(a)
1
3
4
5
+
6
7
8
10
11
9
2
02468101214
Time (min)
5-100% B in 30 min
30
o
C; k* ≈ 15,
R
s
= 0.1
(b)
11
1
3
2
4
5
+
6
7
8
9
10
024681012
Time (min)
1
2
4
5
7
6
3
8
9
10
11
100% ... Section 9.2.2.5: (1) to clean the column between
sample injections, (2) to shorten run time, or (3) to improve separation by adjusting
selectivity for different par...
... acid
(N-Acetylneuraminate)
(Sia)
β-D-N-Acetylgalactosamine
(GalNAc)
β-D-N-Acetylglucosamine
(GlcNAc)
β-D-Galactose
(Gal)
3
H
O
N-C-CH
N-C-CH
3
H
O
H
H
3
-C-N
O
Figure 13. 5 Sugar residues commonly found in glycoproteins.
13. 3 SPECIAL ... Da); 49% acetonitrile-pH-2 .3 buffer. Note the column-packing pore-diameter at top
of each figure; ‘‘ligand coverage’’ (x-axis) is proportional to...
... denaturing conditions [74], for example, between 10 and 35
◦
Cin
Figure 13. 24.
0 5 10 15 20 25 30
10°C
25°C
32 °C
35 °C
40°C
50°C
Figure 13. 24 Effect of temperature on the HIC elution behavior of ... SEPARATIONS
ribonuclease
lysozyme
chymotrypsinogen A
40
30
20
10
C
4
-C
3
OH
C
1
C
3
C
2
Retention
time (min)
Figure 13. 22 Effect of HIC ligand length on protein retention (–C
3...
... CHARACTERISTICS 7 03
CHNHC
O
CH
3
NO
2
NO
2
N
O
NHCCH
2
NHCCH
2
Br
OO
01 530
time (min)
MeOH/AcOH/
TEA
MeOH/
NH
4
AcO
MeOH/TFA/
NH
4
OH
HO
OH
H
N
C(CH
3
)
3
OH
Terbutalin
α= 1 .38
α= 1 .30
α= 1 .30
(a)
(b)
0 132 6
time ... 701
N
H
HN
N
H
N
H
N
H
NH
O
O
O
O
O
O
O
O
O
O
O
O
NH
2
OH
OH
OH
NH
NH
2
OH
Cl
HO
Cl
OH
HO
OH
HO
H
3
C
H
3
C
OH
Vancomycin
A
B
C
O
N
H
N
H
N
H
Cl
N
H
H
O
O
H...
... as
acetonitrile or acetone to a homogenized portion of a vegetable sample allows the
extraction of pesticides into the solvent (step 2 of Fig. 16.7). Subsequent addition
of salt (step 3) leads to ... modern alternative to
Soxhlet extraction; it achieves analyte recoveries equivalent to those from Soxhlet
extraction, but with less solvent and in only 10 to 20 minutes. Sample weigh...
... a result of injector rotor-seal leakage. Push -to- fill autosamplers
(Section 3. 6.2.2, Fig. 3. 21) and most manual injectors make the connection between
the needle and the injector valve with a low-pressure ... cases the sleeve may need to be replaced. Consult the autosampler manual for
specific instructions for your autosampler.
Needle-in-loop autosamplers (Section 3. 6.2 .3, Fig. 3. 22...
... 17.4 .3. 6 first. The following sections
(17.4 .3. 1–17.4 .3. 5) cover each of these symptoms. It may be appropriate at this
point to run the system reference test (Sections 3. 10.1 .3, 17.2.1) to determine ... split and distorted peaks in
Section 17.4.5 .3) ; otherwise, it may be necessary to replace the column.
Before putting the system back into service, consider if steps need to b...