Introduction to Modern Liquid Chromatography, Third Edition part 3 ppsx

Introduction to Modern Liquid Chromatography, Third Edition part 3 ppsx

Introduction to Modern Liquid Chromatography, Third Edition part 3 ppsx

... Polymer Analysis, 6 53 13. 10 .3. 1 Size-Exclusion Chromatography, 6 53 13. 10 .3. 2 Interactive Liquid Chromatography, 6 53 13. 10 .3. 3 Liquid Chromatography under Critical Conditions, 655 13. 10 .3. 4 Other Techniques, ... 730 15.2.1 Columns, 730 15.2.2 Sample Introduction, 731 15.2.2.1 Loop Injectors, 731 15.2.2.2 Pump Injection, 732 15.2 .3 Detectors, 733 15.2 .3. 1 U...
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Introduction to Modern Liquid Chromatography, Third Edition part 13 ppsx

Introduction to Modern Liquid Chromatography, Third Edition part 13 ppsx

... W. Stout, J. J. Destefano, and L. R. Snyder, J. Chromatogr., 282 (19 83) 2 63. 34 . J. H. Knox and H. P. Scott, J. Chromatogr., 282 (19 83) 297. 35 . K. Miyabe, J. Chromatogr. A, 1167 (2007) 161. 36 . ... Snyder and J. J. Kirkland, Introduction to Modern Liquid Chromatography, 2nd ed., Wiley-Interscience, New York, 1979, pp. 34 36 . 46. Reviewer Guidance. Validation of Chromatog...
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Introduction to Modern Liquid Chromatography, Third Edition part 34 ppsx

Introduction to Modern Liquid Chromatography, Third Edition part 34 ppsx

... SAMPLES 02468 Time (min) 024 Time (min) 13 2 4 5 6 024 Time (min) 1 2 3 4 + 5 6 1 2 3 4 5 6 02 Time (min) 1 2 + 3 4 + 5 6 (a) 45% B, 35 °C 2 ≤ k ≤ 13 R s = 0.5 (b) 50% B, 35 °C 1 ≤ k ≤ 7 R s = 0.6 (c) 45% ... naphthalenes. Sample substituents are: 1,1-NHCOCH 3 ; 2,2-SO 2 CH 3 ; 3, 2-OH; 4,1-COCH 3 ; 5,1-NO 2 ;6,2-OCH 3 ; 7, -H (naphthalene); 8,1-SCH 3 ; 9, 1-Cl. Condi...
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Introduction to Modern Liquid Chromatography, Third Edition part 49 ppsx

Introduction to Modern Liquid Chromatography, Third Edition part 49 ppsx

... min 30 o C; k* ≈ 5, R s = 0.1 (a) 1 3 4 5 + 6 7 8 10 11 9 2 02468101214 Time (min) 5-100% B in 30 min 30 o C; k* ≈ 15, R s = 0.1 (b) 11 1 3 2 4 5 + 6 7 8 9 10 024681012 Time (min) 1 2 4 5 7 6 3 8 9 10 11 100% ... Section 9.2.2.5: (1) to clean the column between sample injections, (2) to shorten run time, or (3) to improve separation by adjusting selectivity for different par...
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Introduction to Modern Liquid Chromatography, Third Edition part 63 ppsx

Introduction to Modern Liquid Chromatography, Third Edition part 63 ppsx

... acid (N-Acetylneuraminate) (Sia) β-D-N-Acetylgalactosamine (GalNAc) β-D-N-Acetylglucosamine (GlcNAc) β-D-Galactose (Gal) 3 H O N-C-CH N-C-CH 3 H O H H 3 -C-N O Figure 13. 5 Sugar residues commonly found in glycoproteins. 13. 3 SPECIAL ... Da); 49% acetonitrile-pH-2 .3 buffer. Note the column-packing pore-diameter at top of each figure; ‘‘ligand coverage’’ (x-axis) is proportional to...
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Introduction to Modern Liquid Chromatography, Third Edition part 66 ppsx

Introduction to Modern Liquid Chromatography, Third Edition part 66 ppsx

... denaturing conditions [74], for example, between 10 and 35 ◦ Cin Figure 13. 24. 0 5 10 15 20 25 30 10°C 25°C 32 °C 35 °C 40°C 50°C Figure 13. 24 Effect of temperature on the HIC elution behavior of ... SEPARATIONS ribonuclease lysozyme chymotrypsinogen A 40 30 20 10 C 4 -C 3 OH C 1 C 3 C 2 Retention time (min) Figure 13. 22 Effect of HIC ligand length on protein retention (–C 3...
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Introduction to Modern Liquid Chromatography, Third Edition part 75 ppsx

Introduction to Modern Liquid Chromatography, Third Edition part 75 ppsx

... CHARACTERISTICS 7 03 CHNHC O CH 3 NO 2 NO 2 N O NHCCH 2 NHCCH 2 Br OO 01 530 time (min) MeOH/AcOH/ TEA MeOH/ NH 4 AcO MeOH/TFA/ NH 4 OH HO OH H N C(CH 3 ) 3 OH Terbutalin α= 1 .38 α= 1 .30 α= 1 .30 (a) (b) 0 132 6 time ... 701 N H HN N H N H N H NH O O O O O O O O O O O O NH 2 OH OH OH NH NH 2 OH Cl HO Cl OH HO OH HO H 3 C H 3 C OH Vancomycin A B C O N H N H N H Cl N H H O O H...
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Introduction to Modern Liquid Chromatography, Third Edition part 84 ppsx

Introduction to Modern Liquid Chromatography, Third Edition part 84 ppsx

... as acetonitrile or acetone to a homogenized portion of a vegetable sample allows the extraction of pesticides into the solvent (step 2 of Fig. 16.7). Subsequent addition of salt (step 3) leads to ... modern alternative to Soxhlet extraction; it achieves analyte recoveries equivalent to those from Soxhlet extraction, but with less solvent and in only 10 to 20 minutes. Sample weigh...
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Introduction to Modern Liquid Chromatography, Third Edition part 87 ppsx

Introduction to Modern Liquid Chromatography, Third Edition part 87 ppsx

... a result of injector rotor-seal leakage. Push -to- fill autosamplers (Section 3. 6.2.2, Fig. 3. 21) and most manual injectors make the connection between the needle and the injector valve with a low-pressure ... cases the sleeve may need to be replaced. Consult the autosampler manual for specific instructions for your autosampler. Needle-in-loop autosamplers (Section 3. 6.2 .3, Fig. 3. 22...
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Introduction to Modern Liquid Chromatography, Third Edition part 88 ppsx

Introduction to Modern Liquid Chromatography, Third Edition part 88 ppsx

... 17.4 .3. 6 first. The following sections (17.4 .3. 1–17.4 .3. 5) cover each of these symptoms. It may be appropriate at this point to run the system reference test (Sections 3. 10.1 .3, 17.2.1) to determine ... split and distorted peaks in Section 17.4.5 .3) ; otherwise, it may be necessary to replace the column. Before putting the system back into service, consider if steps need to b...
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