... transfection.PLD2 and a-tubulin were detected by a polyclonal antibody and amouse monoclonal antibody, respectively, followed by goat anti-rabbit secondary IgG conjugated to Alexa 680 and goat ... genomic context: exon-ic miRNA in noncoding transcripts, intronic miRNAsin noncoding transcripts, and intronic miRNAs inprotein-coding transcripts [8,9].Based on the accumulating knowledge on miRNAbiogenesis, ... generation of RNAi forpSM155 and pSM30, we examined the knockdown ofexpression of firefly Luciferase (Luc). HeLa cells weretransfected with the parental and modified vectors con-taining control and...