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Báo cáo khoa học " NMR characterization of the polysaccharidic fraction from Lentinula edodes grown on olive mill waste waters " pdf

Báo cáo khoa học

Báo cáo khoa học " NMR characterization of the polysaccharidic fraction from Lentinula edodes grown on olive mill waste waters " pdf

... NMR characterization of the polysaccharidic fraction from Lentinula edodes grown on olive mill waste waters Umberto Tomati,aMonica Belardinelli,aEmanuela Galli,aValentina Iori,aDonatella ... high-field NMR study of the polysaccharidic fraction extracted from Lentinula edodes mycelium grown on olive mill waste waters is reported. Diffusion-ordered NMR spectroscopy (DOSY) was applied to the polysaccharidic ... and NMR spectroscopy, and the results were the same; therefore,only the analysis of the fraction extracted from the mycelium grown on OMWW is reported here. The GC analysis, performed on the...
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Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx

Tài liệu Báo cáo khoa học: Kinetic characterization of the first step of the ribozyme-catalyzed trans excision-splicing reaction docx

... preventing the second reactionstep (exon ligation). In addition, the product of the first reaction step is actually the intermediate in the fullTES reaction.Substrate binding The rate constant for the ... 8) of the fraction product bound versus ribozymeconcentration [32,33]. For the equilibrium dissociationconstant of the substrate, KdS, an estimated value canbe obtained from the equation ... in the reactionscheme (Fig. 2).Product dissociationFor the fraction of substrates that do undergo the reaction, the resultant products dissociate from the ribozyme relatively slowly on the...
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Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis ppt

Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis ppt

... in the kcatforCys. This implies that the hydroxyl group of Tyr108 of EhMGL1 is actively involved in the b-elimination andc-elimination of the C–S bond, but not the b-elimina-tion of the ... similar to TFM. Three fluorides on the methylcarbon of TFM and a sulfur atom of the thiol group of Hcy may participate in the formation of additionalhydrogen bonds in the catalytic pocket. Although ... Taken together, these findings suggest thatEhMGL1 is responsible for the decomposition and the maintenance of the cellular concentrations of Met andCys, whereas the physiological substrates of EhMGL2under...
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Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot

Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot

... oxidation of the substrate DHO with the quinone cosub-strate was coupled to the reduction of the chromogen 2,6-di-chloroindophenol. The Km of DHO was determined byvarying the concentration of ... (1–1000 lm) at a fixedconcentration (200 lm)ofQD. The Km of QDwas determinedby varying the concentration of QD(0.1–200 lm) at a fixedconcentration (1 m m) of DHO. The additional Kmvalue for2,6-dichloroindophenol ... [13,14], and the transfer of electrons to ubiqui-none, which is part of the respiratory chain. Because of this connection, pyrimidine formation requires asufficient concentration of oxygen in the cells....
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Báo cáo khoa học: Functional characterization of front-end desaturases from trypanosomatids depicts the first polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan ppt

Báo cáo khoa học: Functional characterization of front-end desaturases from trypanosomatids depicts the first polyunsaturated fatty acid biosynthetic pathway from a parasitic protozoan ppt

... mammals, so the biosynthesis of PUFAs goes through the desaturationand elongation of essential FAs taken from the diet,linoleic and a-linolenic (18:3 n-3) acids, which allow the production of n-6 ... and from the alga Thal. pseudonana.DiscussionWe have functionally characterized all front-enddesaturases detected in the genome databases of the three trypanosomatids. The completion of these ... be syn-thesized by L. major, but we cannot anticipate the proportion of each isomer from our heterologousexpression experiments. This ratio will depend on the relative expression of the D6 and...
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Báo cáo khoa học: Biochemical characterization of the major sorghum grain peroxidase pptx

Báo cáo khoa học: Biochemical characterization of the major sorghum grain peroxidase pptx

... 5.5, containing 1 mm CaCl2,in the presence of 2.5 mmH2O2and varying concentrations of hydrogen donor, at25 °C. The final enzyme concentration was 10 nm and the reaction was started by the ... In the presence of Ca2+, the Tm of SPC4is near that of soybean peroxidase, which is one of the most stable plant peroxidases with a Tm of 90 °Cin the presence of calcium [38].In conclusion, ... Ca2+binding onlyslightly increases the activation energy of heat inactiva-tion of SPC4 from 157 ± 12–170 ± 14 kJÆmol)1. The increased stability of SPC4 in the presence of Ca2+ions was confirmed...
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Báo cáo khóa học: Functional characterization of the evolutionarily divergent fern plastocyanin potx

Báo cáo khóa học: Functional characterization of the evolutionarily divergent fern plastocyanin potx

... obtained by heterologous expres-sion in Escherichia c oli. The construction of the syntheticgene, e xpression c onditions, purification protocol and characterization of the recombinant protein will ... first-order c onditions, in w hich the c oncentration of p roteinacceptor, either in the direct reduction by 5-dRfH, or in the interprotein electron transfer, was in large excess over the amount of dRfH ... amplitude of the fast phase of the l atter represen ted up to 40% of the totalamplitude, with a rate constant (kobs) independent of Pcconcentration (data not shown). From the kobsvalues of thisfast...
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Báo cáo khóa học: Structural characterization of the human Nogo-A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration ppt

Báo cáo khóa học: Structural characterization of the human Nogo-A functional domains Solution structure of Nogo-40, a Nogo-66 receptor antagonist enhancing injured spinal cord regeneration ppt

... enaturin g con ditions (in the presence of 8Murea); lane6, elution 3 under denaturing conditions (in the presence of 8Murea); lane 7, elution 4 under denaturing conditions (in the presence of 8Murea);lane ... Interestingly, with the introduction of meth anol, the CD spectra of Nogo-40undergo dramatic changes. The CD spectra of Nogo-40 in the presence of methanol at a concentration of 74% orabove show one positive ... ecloned and expressed the two functional regions of humanNogo-A and performed structural characterization by CDCorrespondence to J. Song, Department of Biochemistry, NationalUniversity of...
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Báo cáo khoa học: Structural characterization of photosystem II complex from red alga Porphyridium cruentum retaining extrinsic subunits of the oxygen-evolving complex docx

Báo cáo khoa học: Structural characterization of photosystem II complex from red alga Porphyridium cruentum retaining extrinsic subunits of the oxygen-evolving complex docx

... centrifugation of solubilizedthylakoid membranes (fractions A–C, Fig. 1A). Fraction Ain the upper part of the gradient contained 24% of totalchlorophyll content and the rest of the chlorophyll ... height of about 9 nm is constant in all the projections. As the length s of the side-views c orrespond well with the length and width of particle in the top-view projection, the distinct lengths of the ... variations in the appearance of the protrusions. The distances between the protrusion s areproportional to the lengths o f particles, which demonstratean overlap of the extrinsic subunits in the...
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Báo cáo khoa học: NMR structure of the thromboxane A2 receptor ligand recognition pocket pot

Báo cáo khoa học: NMR structure of the thromboxane A2 receptor ligand recognition pocket pot

... recognition site on the extracellulardomain. The second step will then be the deposition of the ligand into the TM pocket causing the conformationchange of the receptor and triggering the coupling of ... conformation movementand defining the final conformation of the eLPs. Thesestudies were limited to the three eLP domains. The conformation of the eLPs in the simulated reduced form of the TP receptor ... ecognitionpocket. The eLP2conformation is dynamic a nd regulatedby the oxidation and reduction of the d isulfide bond, whichaffects ligand docking in the initial recognition. The reducedform of the...
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