... Lys at position 52 and Arg at position 54 or 55 (Lys-Xaa-Arg or Lys-Xaa-Xaa-Arg) are conserved among all except PSLV. Gly atposition 77 is conserved among all except tobraviruses.The secondary ... purposes)mixture containing 5 mM ATP, CTP, UTP, and GTP (Phar-macia-Pfizer, Mississauga, Ontario, Canada), 0.7 µl of T7polymerase (Ambion), and nuclease-free water to a finalvolume of 25 µl. The reactions ... lk-gCvnevtvlgHetCsigHanklrkqvadmvg vtrrCaen-nCgwfvCviin-dft tobra PeRSV mtkCa lp-eCeentqkn-qmtCsmkHankynrylaskfd vkrkCeCk-nCgwfpaisvqpdy tobra PEBV mkCa vs-tCeveaqsn-kftCsmkCankynrHlaekys ikrkCeCv-nCgwypaievradf...