... culture system formulated initially for SSCexpansion, containing LIF, putrescine, EGF, GDNF, bFGF,insulin and transferrin. The proliferating cells that resultedwere described as forming compact ... detectable by in situ BrdU immunocy-tochemistry, in the neonatal mouse ovary. The distinct,nuclear staining for BrdU in the oocyte of Fig ure two ‘e’ of Johnson et al. [7], and in oocytes in Figure ... (A) ma intenance of self-tolerance to ovarianantigens during homeostasis; (B) after ovotoxic CT,induction of apoptosis in follicular cells leading to fail-ure of tolerance, induction of autoimmunity...